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首页> 外文期刊>World Journal of Gastroenterology >Resveratrol engages selective apoptotic signals in gastric adenocarcinoma cells.
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Resveratrol engages selective apoptotic signals in gastric adenocarcinoma cells.

机译：白藜芦醇参与胃腺癌细胞中的选择性凋亡信号。

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AIM: To investigate the intracellular apoptotic signals engaged by resveratrol in three gastric adenocarcinoma cancer cell lines, two of which (AGS and SNU-1) express p53 and one (KATO-III) with deleted p53. METHODS: Nuclear fragmentation was used to quanti-tate apoptotic cells; caspase activity was determined by photometric detection of cleaved substrates; formation of oxidized cytochrome C was used to measure cytochrome C activity, and Western blot analysis was used to determine protein expression. RESULTS: Gastric cancer cells, irrespective of their p53 status, responded to resveratrol with fragmentation of DNA and cleavage of nuclear lamins A and B and PARP. Resveratrol, however, has no effect on mitochondria-associated apoptotic proteins Bcl-2, Bcl-xl, Bax, Bid or Smac/Diablo, and did not promote sub-cellular redistribution of cytochrome C, indicating that resveratrol-induced apoptosis of gastric carcinoma cells does not require breakdown of mitochondrial membrane integrity. Resveratrol up-regulated p53 protein in SNU-1 and AGS cells but there was a difference in response of intracellular apoptotic signals between these cell lines. SNU-1 cells responded to resveratrol treatment with down-regulation of survivin, whereas in AGS and KATO-III cells resveratrol stimulated caspase 3 and cytochrome C oxidase activities. CONCLUSION: These findings indicate that even within a specific cancer the intracellular apoptotic signals engaged by resveratrol are cell type dependent and suggest that such differences may be related to differentiation or lack of differentiation of these cells.
机译：目的：研究白藜芦醇在三种胃腺癌细胞系中的细胞内凋亡信号，其中两种（AGS和SNU-1）表达p53，一种（KATO-III）表达p53。方法：核碎裂用于定量凋亡细胞。半胱天冬酶活性通过光度检测裂解的底物来确定；氧化的细胞色素C的形成用于测量细胞色素C的活性，蛋白质印迹分析用于确定蛋白质的表达。结果：胃癌细胞，无论其p53状态如何，对白藜芦醇的反应都是DNA断裂以及核纤层蛋白A和B和PA​​RP的裂解。然而，白藜芦醇对线粒体相关的凋亡蛋白Bcl-2，Bcl-xl，Bax，Bid或Smac / Diablo没有影响，并且不促进细胞色素C的亚细胞再分布，表明白藜芦醇诱导的胃癌细胞凋亡。细胞不需要破坏线粒体膜的完整性。白藜芦醇在SNU-1和AGS细胞中上调了p53蛋白，但这些细胞系之间的细胞内凋亡信号的响应存在差异。 SNU-1细胞对surverin的下调对白藜芦醇治疗有反应，而在AGS和KATO-III细胞中，白藜芦醇刺激半胱天冬酶3和细胞色素C氧化酶活性。结论：这些发现表明，即使在特定的癌症中，白藜芦醇参与的细胞内凋亡信号也依赖于细胞类型，并表明这种差异可能与这些细胞的分化或缺乏分化有关。

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