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首页> 外文期刊>World Journal of Gastroenterology >Reduced expression of Ca(2+)-regulating proteins in the upper gastrointestinal tract of patients with achalasia.
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Reduced expression of Ca(2+)-regulating proteins in the upper gastrointestinal tract of patients with achalasia.

机译：门失弛缓症患者上消化道中Ca（2+）调节蛋白的表达减少。

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AIM: To compare expression of Ca(2+)-regulating proteins in upper gastrointestinal (GI) tract of achalasia patients and healthy volunteers and to elucidate their role in achalasia. METHODS: Sarcoplasmic reticulum Ca(2+) ATPase (SERCA) isoforms 2a and 2b, phospholamban (PLB), calsequestrin (CSQ), and calreticulin (CRT) were assessed by quantitative Western blotting in esophagus and heart of rats, rabbits, and humans. Furthermore, expression profiles of these proteins in biopsies of lower esophageal sphincter and esophagus from patients with achalasia and healthy volunteers were analyzed. RESULTS: SERCA 2a protein expression was much higher in human heart (cardiac ventricle) compared to esophagus. However, SERCA 2b was expressed predominantly in the esophagus. The highest CRT expression was noted in the human esophagus, while PLB, although highly expressed in the heart, was below our detection limit in upper GI tissue. Compared to healthy controls, CSQ and CRT expression in lower esophageal sphincter anddistal esophageal body were significantly reduced in patients with achalasia (P < 0.05). CONCLUSION: PLB in the human esophagus might be of lesser importance for regulation of SERCA than in heart. Lower expression of Ca(2+) storage proteins (CSQ and CRT) might contribute to increased lower esophageal sphincter pressure in achalasia, possibly by increasing free intracellular Ca(2+).
机译：目的：比较失弛缓症患者和健康志愿者在上消化道（GI）中表达Ca（2+）的蛋白的表达，并阐明其在失弛缓症中的作用。方法：通过定量Western印迹法对大鼠，兔和人的食道和心脏中的肌浆网C​​a（2+）ATPase（SERCA）亚型2a和2b，磷酸lamban（PLB），钙网蛋白（CSQ）和钙网蛋白（CRT）进行了评估。 。此外，分析了这些蛋白在门失弛缓症患者和健康志愿者的食管下括约肌和食道活检中的表达谱。结果：与食道相比，人心脏（心室）中的SERCA 2a蛋白表达高得多。但是，SERCA 2b主要在食道中表达。在人的食道中，CRT的表达最高，而PLB虽然在心脏中高表达，但低于我们在上消化道组织中的检测极限。与健康对照组相比，门失弛缓症患者的下食管括约肌和食管远端CSQ和CRT表达明显降低（P <0.05）。结论：人食道中的PLB对SERCA的调节作用可能不如对心脏重要。 Ca（2+）存储蛋白（CSQ和CRT）的较低表达可能有助于提高门失弛缓性食管括约肌压力，可能是通过增加游离细胞内Ca（2+）引起的。

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