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首页> 外文期刊>World Journal of Gastroenterology >Prevention of core cell damage in isolated islets of Langerhans by low temperature preconditioning.
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Prevention of core cell damage in isolated islets of Langerhans by low temperature preconditioning.

机译：通过低温预处理防止孤立的胰岛中的核心细胞损伤。

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AIM: To study the core cell damage in isolated islets of Langerhans and its prevention by low temperature preconditioning (26 degrees). METHODS: Islets were cultured at 37 degrees for 7-14 d after isolation, and then at 26 degrees for 2, 4 and 7 d before additional culture at 37 degrees for another 7 d. Core cell damage in the isolated islets was monitored by video-microscopy and analyzed quantitatively by use of a computer-assisted image analysis system. The analysis included daily measurement of the diameter and the area of the isolated islets and the area of the core cell damage that developed in those islets over time during culture. Histology and TdT-mediated dUTP-biotin nick end labeling (TUNEL) assay were used to characterize the cell damage and to monitor islet function. RESULTS: Microscopic analysis showed that during the 7 to 14 d of culture at 37 degrees, core cell damage occurred in the larger islets with diameters >200 microm, which included both necrotic and apoptotic cell death. Low temperature (26 degrees) culture could prevent core cell damage of isolated islets. The 7-d culture procedure at 26 degrees could inhibit most of the core cell (excluding diameters >300 microm) damages when the islets were re-warmed at 37 degrees. CONCLUSION: Our results indicate that core cell damage within isolated islets of Langerhans correlates with the size of islets. Low temperature (26 degrees) culture can prevent core cell damage in isolated islets, and successfully precondition these islets for incubation at 37 degrees. These novel findings may help to understand the pathophysiology of early loss of islet tissue after transplantation, and may provide a new strategy to improve graft function in the clinical setting of islet transplantation.
机译：目的：研究朗格汉斯岛离体胰岛中的核心细胞损伤及其低温预处理（26度）的预防作用。方法：分离后，将胰岛在37度下培养7-14 d，然后在26度下分别培养2、4和7 d，然后再在37度下再培养7 d。通过视频显微镜监控分离的胰岛中的核心细胞损伤，并使用计算机辅助图像分析系统进行定量分析。分析包括每日测量分离的胰岛的直径和面积，以及在培养过程中随着时间的推移在这些胰岛中形成的核心细胞损伤的面积。组织学和TdT介导的dUTP-生物素缺口末端标记法（TUNEL）用于表征细胞损伤并监测胰岛功能。结果：显微分析表明，在37度培养的7至14天期间，直径大于200微米的较大胰岛发生了核心细胞损伤，包括坏死性和凋亡性细胞死亡。低温（26度）培养可以预防离体胰岛的核心细胞损伤。当在37度重新加热胰岛时，在26度进行7 d培养程序可以抑制大多数核心细胞（直径> 300 microm除外）的损害。结论：我们的结果表明，孤立的胰岛中的核心细胞损伤与胰岛的大小有关。低温（26度）培养可以防止分离的胰岛中的核心细胞受损，并成功地对这些胰岛进行预处理，使其可以在37度下孵育。这些新颖的发现可能有助于了解胰岛组织移植后早期丢失的病理生理，并可能为改善胰岛移植临床环境中的移植功能提供新的策略。

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