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首页> 外文期刊>World Journal of Gastroenterology >Effect of glutamate on inflammatory responses of intestine and brain after focal cerebral ischemia.
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Effect of glutamate on inflammatory responses of intestine and brain after focal cerebral ischemia.

机译：谷氨酸对局灶性脑缺血后肠道和脑部炎症反应的影响。

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AIM: To study the modulation of glutamate on post-ischemic intestinal and cerebral inflammatory responses in a ischemic and excitotoxic rat model. METHODS: Adult male rats were subjected to bilateral carotid artery occlusion for 15 min and injection of monosodium glutamate intraperitoneally, to decapitate them at selected time points. Tumor necrosis factor alpha (TNF-alpha) level and nuclear factor kappa B (NF-kappaB) activity were determined by enzyme-linked immunosorbant assay (ELISA) and electrophoretic mobility shift assay (EMSA), respectively. Hemodynamic parameters were monitored continuously during the whole process of cerebral ischemia and reperfusion. RESULTS: Monosodium glutamate (MSG) treated rats displayed statistically significant high levels of TNF-alpha in cerebral and intestinal tissues within the first 6 h of ischemia. The rats with cerebral ischemia showed a minor decrease of TNF-alpha production in cerebral and intestinal tissues. The rats with cerebral ischemia and treated with MSG displayed statistically significant low levels of TNF-alpha in cerebral and intestinal tissues. These results correlated significantly with NF-kappaB production calculated at the same intervals. During experiment, the mean blood pressure and heart rates in all groups were stable. CONCLUSION: Glutamate is involved in the mechanism of intestinal and cerebral inflammation responses. The effects of glutamate on cerebral and intestinal inflammatory responses after ischemia are up-regulated at the transcriptional level, through the NF-kappaB signal transduction pathway.
机译：目的：研究谷氨酸在缺血和兴奋性毒性大鼠模型中对缺血后肠道和脑部炎症反应的调节作用。方法：成年雄性大鼠进行双侧颈动脉闭塞15分钟并腹膜内注射谷氨酸钠，在选定的时间点使他们断头。分别通过酶联免疫吸附测定（ELISA）和电泳迁移率变动测定（EMSA）测定肿瘤坏死因子α（TNF-α）水平和核因子κB（NF-κB）活性。在脑缺血和再灌注的整个过程中连续监测血流动力学参数。结果：谷氨酸钠（MSG）处理的大鼠在缺血的前6小时内在脑和肠组织中显示出统计学上显着的高水平的TNF-α。有脑缺血的大鼠在脑和肠组织中的TNF-α产量略有下降。患有脑缺血并用味精治疗的大鼠在脑和肠组织中显示出统计学上显着的低水平TNF-α水平。这些结果与以相同间隔计算的NF-κB产生显着相关。在实验过程中，所有组的平均血压和心率均保持稳定。结论：谷氨酸参与肠道和脑部炎症反应的机制。通过NF-κB信号转导途径，谷氨酸对局部缺血后脑和肠道炎症反应的影响在转录水平上调。

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