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首页> 外文期刊>World Journal of Gastroenterology >Enhanced production of leptin in gastric fundic mucosa with Helicobacter pylori infection.
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Enhanced production of leptin in gastric fundic mucosa with Helicobacter pylori infection.

机译：幽门螺杆菌感染可增强胃底黏膜中瘦素的产生。

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AIM: To determine the concentrations of leptin in plasma and gastric fundic mucosa in humans, with reference to Helicobacter pylori (H pylori) infection, and their association with gastric mucosal levels of interleukin (IL)-1beta, IL-6 and IL-8. METHODS: Plasma leptin concentrations were determined in 135 outpatients with non-ulcer dyspepsia, consisting of 95 H pylori-infected and 40 uninfected subjects, and 13 patients before and after cure of the infection with anti-H pylori regimen. Using biopsy samples that were endoscopically obtained from the middle corpus along the greater curvature, gastric leptin contents were measured by radioimmunoassay and the mucosal concentrations of IL-1beta, IL-6 and IL-8 were measured by enzyme linked immunosorbent assay. We also analysed the expression of leptin in the fundic mucosa by reverse transcriptase-polymerase chain reaction (RT-PCR) and immunohistochemistry. RESULTS: The mucosal levels of leptin in the fundic mucosa of H pylori-infected patients were significantly higher than those of uninfected patients. The amount of gastric leptin correlated positively with the mucosal levels of IL-1beta and IL-6, but not IL-8. Circulating leptin correlated with body mass index, but not with H pylori status, and there was no change in plasma leptin levels following cure of the infection. Leptin immunoreactive cells were noted in the lower half of the fundic glands, and its expression of messenger ribonucleic acid in the oxyntic mucosa was detected by RT-PCR. CONCLUSION: Leptin production is enhanced in H pylori-infected gastric mucosa. Gastric leptin may be involved in immune and inflammatory response during H pylori infection, through interaction with proinflammatory cytokines.
机译：目的：确定人血浆和胃底胃黏膜中瘦素的浓度，以幽门螺杆菌（H pylori）感染为参考，并将其与胃黏膜白介素（IL）-1beta，IL-6和IL-8水平相关联。方法：测定135例非溃疡性消化不良患者的血浆瘦素浓度，包括95例幽门螺杆菌感染者和40例未感染者，以及13例抗H幽门螺杆菌感染治愈前后的患者。使用从内体沿较大曲率在内窥镜下获得的活检样品，通过放射免疫测定法测定胃瘦素含量，并通过酶联免疫吸附测定法测定IL-1β，IL-6和IL-8的粘膜浓度。我们还通过逆转录聚合酶链反应（RT-PCR）和免疫组织化学分析了瘦素在胃黏膜中的表达。结果：幽门螺杆菌感染患者胃底黏膜中的瘦素水平明显高于未感染者。胃瘦素的量与粘膜水平的IL-1β和IL-6呈正相关，但与IL-8无关。循环中的瘦素与体重指数相关，但与幽门螺杆菌状态无关，并且感染治愈后血浆瘦素水平没有变化。在眼底下半部发现了瘦素免疫反应性细胞，并通过RT-PCR检测了其在催产性粘膜中的信使核糖核酸表达。结论：幽门螺杆菌感染的胃黏膜中瘦素的产生增加。胃瘦素可能通过与促炎细胞因子的相互作用而参与幽门螺杆菌感染期间的免疫和炎症反应。

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