• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>World Journal of Gastroenterology >Alcohol dehydrogenase: a potential new marker for diagnosis of intestinal ischemia using rat as a model.
【24h】

Alcohol dehydrogenase: a potential new marker for diagnosis of intestinal ischemia using rat as a model.

机译：酒精脱氢酶：一种以大鼠为模型诊断肠道缺血的潜在新标记。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

AIM: Intestinal ischemia (Ii) is an abdominal emergency due to blockade of the superior mesenteric artery resulting in 60-100% mortality if diagnosed late. Changes in several biochemical parameters such as D (-)-lactate, Creatinine kinase isoenzymes and lactate dehydrogenase suggested for early diagnosis, lack specificity and sensitivity. Therefore a biochemical parameter with greater sensitivity needs to be identified. METHODS: Wistar male rats were randomly assigned into two groups; control sham operated (n = 24) and ischemic test (n = 24) group. Superior mesenteric arterial occlusion was performed in the ischemic test group for 1 h. Alcohol dehydrogenase (ADH) was estimated in blood from portal vein, right ventricle of heart, dorsal aorta (DA) and inferior vena cava (IVC). The Serum glutamic acid pyruvate transaminase (SGPT) was also estimated in blood from portal vein and right ventricle of heart. RESULTS: A significant increase (P<0.001) in the levels of ADH in both portal blood as well as heart blood of the test group (232.72+/-99.45 EU and 250.85+/-95.14 EU, respectively) as compared to the control group (46.39+/-21.69 EU and 65.38+/-30.55 EU, respectively) were observed. Similarly, increased levels of ADH were observed in blood samples withdrawn from DA and IVC in test animals (319.52+/-80.14 EU and 363.90+/-120.68 EU, respectively) as compared to the control group (67.68+/-63.22 EU and 72.50+/-58.45 EU, respectively). However, in test animals there was significant increase in SGPT in portal blood (P = 0.054) without much increase in heart blood. CONCLUSION: Significant increase in the levels of ADH in portal and heart blood within 1 h of SMA occlusion without increase in SGPT in heart blood, suggests that the origin of ADH is from ischemic intestine and not from liver. Similarly, raised ADH levels were found in DA and IVC as well. IVC blood does represent peripheral blood sample. A raised level of ADH in test animals confirms it to be a potential marker in the early diagnosis of Ii.
机译：目的：肠缺血（Ii）是肠系膜上动脉阻塞所致的腹部紧急情况，如果诊断得晚，将导致60-100％的死亡率。有人建议对D（-）-乳酸，肌酐激酶同工酶和乳酸脱氢酶等几种生化参数的变化进行早期诊断，但缺乏特异性和敏感性。因此，需要确定具有更高灵敏度的生化参数。方法：Wistar雄性大鼠随机分为两组。对照假手术组（n = 24）和缺血测试组（n = 24）。在缺血测试组中进行肠系膜上动脉阻塞1 h。在门静脉，心脏右心室，背主动脉（DA）和下腔静脉（IVC）的血液中估计了乙醇脱氢酶（ADH）。还估计了来自门静脉和心脏右心室的血液中的血清谷氨酸丙酮酸转氨酶（SGPT）。结果：与对照组相比，测试组的门静脉和心脏血液中的ADH水平显着增加（P <0.001）（分别为232.72 +/- 99.45 EU和250.85 +/- 95.14 EU）。观察组（分别为46.39 +/- 21.69 EU和65.38 +/- 30.55 EU）。相似地，与对照组（67.68 +/- 63.22 EU和分别为72.50 +/- 58.45 EU）。但是，在试验动物中，门静脉血中SGPT显着增加（P = 0.054），而心脏血液中却没有太多增加。结论：SMA闭塞后1小时内，门静脉和心脏血液中的ADH水平显着增加，而心脏血液中的SGPT却没有升高，这表明ADH的起源是缺血性肠而非肝脏。同样，在DA和IVC中也发现升高的ADH水平。 IVC血液确实代表外周血样本。实验动物中ADH水平升高证实它是II早期诊断的潜在标志物。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号