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首页> 外文期刊>World Journal of Gastroenterology >Effects of STI571 and p27 gene clone on proliferation and apoptosis of K562 cells.
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Effects of STI571 and p27 gene clone on proliferation and apoptosis of K562 cells.

机译：STI571和p27基因克隆对K562细胞增殖和凋亡的影响。

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摘要

AIM: To investigate the combined effect of STI571 and p27 gene clone on the regulation of proliferation, cell cycle and apoptosis of K562 cell line. METHODS: p27 gene was obtained by RT-PCR, and its sequence was approved to be correct. Then p27-pcDNA3.1 vector was constructed and transfected into K562 cell line. p27-pcDNA3.1-K562 cell clone was screened by G418 after transfection, p27 protein was identified by Western blot. MTT was used to detect the survival rate of the cell. Flow cytometry was used to detect cell cycle and apoptosis index. RESULTS: The expression of p27 protein could be detected by Western blot in p27-pcDNA3.1-K562 cells. A strong inhibition of cell proliferation was observed in p27-pcDNA3.1-K562 cells as compared with that of the control (pcDNA3.1-K562 cells). The cells at G0/G1 phase were significantly increased, and cells at S phase were greatly declined. The apoptosis index was increased greatly after p27-pcDNA3.1-K562 cells were treated with STI571, and survival rate of the cellwas markedly declined (0.35-0.58, P<0.05-0.048 vs STI571-K562 cell, 0.35-0.72, P<0.01-0.001 vs p27-K562 cell). CONCLUSION: p27 and STI571 have a synergistic action on inhibition of proliferation and induction of apoptosis on K562 cells.
机译：目的：探讨STI571和p27基因克隆对K562细胞增殖，细胞周期和凋亡的调控作用。方法：通过RT-PCR获得p27基因，序列正确。然后构建p27-pcDNA3.1载体并将其转染到K562细胞系中。转染后经G418筛选p27-pcDNA3.1-K562细胞克隆，Western blot鉴定p27蛋白。使用MTT检测细胞的存活率。流式细胞仪用于检测细胞周期和凋亡指数。结果：Western blot检测p27-pcDNA3.1-K562细胞中p27蛋白的表达。与对照（pcDNA3.1-K562细胞）相比，在p27-pcDNA3.1-K562细胞中观察到了对细胞增殖的强烈抑制。 G0 / G1期的细胞明显增加，而S期的细胞大大减少。 STI571处理p27-pcDNA3.1-K562细胞后，凋亡指数显着增加，存活率明显下降（0.35-0.58，P <0.05-0.048，相对于STI571-K562细胞，0.35-0.72，P < 0.01-0.001 vs p27-K562细胞）。结论：p27和STI571在抑制K562细胞的增殖和诱导细胞凋亡方面具有协同作用。

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