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首页> 外文期刊>World Journal of Gastroenterology >Effects of total glucosides of peony on immunological hepatic fibrosis in rats.
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Effects of total glucosides of peony on immunological hepatic fibrosis in rats.

机译：牡丹总苷对大鼠免疫性肝纤维化的影响。

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AIM: To study the effects of total glucosides of peony (TGP) on immunological hepatic fibrosis induced by human albumin in rats. METHODS: Sixty adult male Sprague-Dawley rats were randomly divided into: Normal group, model group, TGP (60 and 120 mg/kg) treatment groups and colchicines (0.1 mg/kg) treatment group. On the day before the rats were killed, those in TGP or colchicine groups received TGP or colchicine as above from the first day of tail vein injection of human albumin. The rats in normal and model groups were only administered with the same volume of vehicle. At the end of the 16th wk, rats in each group were killed. Blood and tissue specimens were taken. Levels of alanine aminotransferase (ALT), aspartate aminotr-ansferase (AST), nitric oxide (NO), content of malondi-aldehyde (MDA), activity of superoxide dismutase (SOD) and glutathione peroxidase (GSH-px), were measured by biochemical methods. Serum procollagen type III (PC III) and laminin (LN) were determined by radioimmunoassay. Liver collagen level was determined by measuring hydroxyproline content in fresh liver samples. Hepatic tissue sections were stained with hematoxylin-eosin and examined under a light microscope. RESULTS: Histological results showed that TGP improved the human albumin-induced alterations in the liver structure, alleviated lobular necrosis and significantly lowered collagen content. The antifibrotic effect of TGP was also confirmed by decreased serum content of LN and PCIII in TGP-treated group. Moreover, the treatment with TGP effectively reduced the hydroxyproline content in liver homogenates. However, the level of ALT and AST increased in fibrotic rat but had no significance compared with normal control, whereas the ratio of A/G decreased without significance. TGP had no effect on level of ALT, AST and the ratio of A/G. Furthermore, TGP treatment significantly blocked the increase in MDA and NO, asso-ciated with a partial elevation in liver total antioxidant capacity including SOD and GSH-px. CONCLUSION: TGP has beneficial effects on hepatic fibrosis in rats by inhibition of collagen synthesis and decreasing oxidative stress.
机译：目的：研究牡丹总苷（TGP）对人白蛋白诱导的大鼠免疫性肝纤维化的影响。方法：将60只成年雄性Sprague-Dawley大鼠随机分为正常组，模型组，TGP（60和120 mg / kg）治疗组和秋水仙碱（0.1 mg / kg）治疗组。在处死大鼠的前一天，从人静脉白蛋白尾静脉注射的第一天开始，TGP或秋水仙碱组的大鼠接受上述的TGP或秋水仙碱。正常组和模型组的大鼠仅施用相同体积的载体。在第16周结束时，将每组大鼠杀死。采集血液和组织标本。丙氨酸氨基转移酶（ALT），天冬氨酸氨基转移酶（AST），一氧化氮（NO），丙二醛（MDA）含量，超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GSH-px）的水平通过生化方法。通过放射免疫测定法测定血清III型胶原原（PC III）和层粘连蛋白（LN）。通过测量新鲜肝脏样品中的羟脯氨酸含量来确定肝脏胶原蛋白水平。肝组织切片用苏木精-曙红染色，并在光学显微镜下检查。结果：组织学结果表明，TGP改善了人白蛋白引起的肝脏结构改变，减轻了小叶坏死并显着降低了胶原蛋白含量。 TGP治疗组的血清LN和PCIII含量降低也证实了TGP的抗纤维化作用。此外，TGP处理有效降低了肝脏匀浆中羟脯氨酸的含量。然而，与正常对照组相比，纤维化大鼠的ALT和AST水平升高，但无统计学意义，而A / G比降低则无统计学意义。 TGP对ALT，AST和A / G比没有影响。此外，TGP治疗显着阻止了MDA和NO的增加，与肝脏总抗氧化能力（包括SOD和GSH-px）的部分升高有关。结论：TGP可通过抑制胶原合成和降低氧化应激对大鼠肝纤维化产生有益作用。

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