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首页> 外文期刊>World Journal of Gastroenterology >Comparison of six human anti-transglutaminase ELISA-tests in the diagnosis of celiac disease in the Saharawi population
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Comparison of six human anti-transglutaminase ELISA-tests in the diagnosis of celiac disease in the Saharawi population

机译：六种人类抗转谷氨酰胺酶联免疫吸附测定（ELISA）检测在撒哈拉维人群乳糜泻诊断中的比较

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AIM: Celiac disease (CD) is an enteropathic disorder very prevalent in Saharawi people. Our aim was to investigate the diagnostic accuracy of six human tissue transglutaminase (tTG) based ELISA tests in Saharawi CD patients. METHODS: Fifty-two CD patients and 23 controls were selected from the Saharawi refugee camps in Tinduf. CD patients were divided into two groups according to their anti-endomysium (EmA) status: 41 EmA positive and 11 EmA negative. Sera from patients and controls were tested for human tTG using six commercial ELISA kits. We used receiver operating characteristics (ROC) curves and areas under the curve to compare the diagnostic accuracies of the six assays. RESULTS: In general, there are differences in the sensitivity and specificity of the human tTG ELISA assays used. Diagnostic accuracy of tests was significantly improved by adjusting the cut-off thresholds according to ROC plot analysis; the correction of the cut-off with the employment of the ROC curve analysis modifies the decision limit in more than 50% in five of the six kits evaluated. CONCLUSION: Some of the human tTG ELISAs used in this study have a diagnostic accuracy similar to EmA determination for diagnosis of CD in Saharawi people. However, it is necessary to select the assay with a higher sensitivity and specificity, and recalculate the cut-off threshold using samples from the referral population.
机译：目的：腹腔疾病（CD）是一种在撒哈拉维人中非常普遍的肠病。我们的目的是调查六种基于人体组织转谷氨酰胺酶（tTG）的ELISA检测对撒哈拉维CD患者的诊断准确性。方法：从廷杜夫市的撒哈拉难民营中选出52名CD患者和23名对照。 CD患者根据其抗内胚层（EmA）状况分为两组：41个EmA阳性和11个EmA阴性。使用六种商用ELISA试剂盒测试了患者和对照组的血清中的人tTG。我们使用接收器工作特性（ROC）曲线和曲线下的面积来比较这六种检测方法的诊断准确性。结果：通常，所使用的人tTG ELISA分析的敏感性和特异性存在差异。通过根据ROC图分析调整截止阈值，可以显着提高测试的诊断准确性；通过使用ROC曲线分析对截止值进行校正，可以在六个评估套件中的五个套件中将决策限制修改为超过50％。结论：本研究中使用的某些人类tTG ELISA具有与EmA测定相似的诊断准确性，可用于诊断撒哈拉维人的CD。但是，有必要选择具有更高灵敏度和特异性的检测方法，并使用转诊人群的样品重新计算截断阈值。

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