• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>World Journal of Gastroenterology >Liver-specific gene expression in mesenchymal stem cells is induced by liver cells
【24h】

Liver-specific gene expression in mesenchymal stem cells is induced by liver cells

机译：肝细胞诱导间充质干细胞中的肝特异性基因表达

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

AIM: The origin of putative liver cells from distinct bone marrow stem cells, e.g. hematopoietic stem cells or multipotent adult progenitor cells was found in recent in vitro studies. Cell culture experiments revealed a key role of growth factors for the induction of liver-specific genes in stem cell cultures. We investigated the potential of rat mesenchymal stem cells (MSC) from bone marrow to differentiate into hepatocytic cells in vitro. Furthermore, we assessed the influence of cocultured liver cells on induction of liver-specific gene expression. METHODS: Mesenchymal stem cells were marked with green fluorescent protein (GFP) by retroviral gene transduction. Clonal marked MSC were either cultured under liver stimulating conditions using fibronectin-coated culture dishes and medium supplemented with SCF, HGF, EGF, and FGF-4 alone, or in presence of freshly isolated rat liver cells. Cells in cocultures were harvested and GFP+ or GFP- cells were separated using fluorescence activated cell sorting. RT-PCR analysis for the stem cell marker Thy1 and the hepatocytic markers CK-18, albumin, CK-19, and AFP was performed in the different cell populations. RESULTS: Under the specified culture conditions, rat MSC cocultured with liver cells expressed albumin-, CK-18, CK-19, and AFP-RNA over 3 weeks, whereas MSC cultured alone did not show liver specific gene expression. CONCLUSION: The results indicate that (1) rat MSC from bone marrow can differentiate towards hepatocytic lineage In vitro, and (2) that the microenvironment plays a decisive role for the induction of hepatic differentiation
机译：目的：推定的肝细胞起源于不同的骨髓干细胞，例如在最近的体外研究中发现了造血干细胞或多能成年祖细胞。细胞培养实验揭示了生长因子在干细胞培养中诱导肝特异性基因的关键作用。我们调查了大鼠骨髓间充质干细胞（MSC）体外分化为肝细胞的潜力。此外，我们评估了共培养的肝细胞对诱导肝特异性基因表达的影响。方法：通过逆转录病毒基因转导将间充质干细胞标记为绿色荧光蛋白（GFP）。在肝刺激条件下，使用纤连蛋白包被的培养皿和单独添加了SCF，HGF，EGF和FGF-4的培养基，或者在新鲜分离的大鼠肝细胞存在下，培养克隆标记的MSC。收获共培养物中的细胞，并使用荧光激活细胞分选术分离GFP +或GFP-细胞。在不同的细胞群中进行了干细胞标志物Thy1和肝细胞标志物CK-18，白蛋白，CK-19和AFP的RT-PCR分析。结果：在特定的培养条件下，与肝细胞共培养的大鼠MSC在3周内表达白蛋白，CK-18，CK-19和AFP-RNA，而单独培养的MSC没有显示肝特异性基因表达。结论：结果表明：（1）大鼠骨髓间充质干细胞可以在体外向肝细胞谱系分化，（2）微环境在诱导肝细胞分化中起决定性作用

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号