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首页> 外文期刊>World Journal of Gastroenterology >Apoptosis of pancreatic cancer BXPC-3 cells induced by indole-3-acetic acid in combination with horseradish peroxidase
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Apoptosis of pancreatic cancer BXPC-3 cells induced by indole-3-acetic acid in combination with horseradish peroxidase

机译：吲哚-3-乙酸联合辣根过氧化物酶诱导胰腺癌BXPC-3细胞凋亡

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摘要

AM: To explore the mechanisms underlying the apoptosis of human pancreatic cancer BXPC-3 cells induced by indole-3-acetic acid (IAA) in combination with horseradish peroxidase (HRP). METHODS: BXPC-3 cells derived from human pancreatic cancer were exposed to 40 or 80 μmol/L IAA and 1.2 μg/mL HRP at different times. Then, MTT assay was used to detect the cell proliferation. Flow cytometry was performed to analyze cell cycle. Terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling assay was used to detect apoptosis. 2,7-Dichlorofluorescin diacetate uptake was measured by confocal microscopy to determine free radicals. Level of malondialdehyde (MDA) and activity of superoxide dismutase (SOD) were measured by biochemical methods. RESULTS: IAA/HRP initiated growth inhibition of BXPC-3 cells in a dose- and time-dependent manner. Flow cytometry revealed that the cells treated for 48 h were arrested at G_1/G_0. After exposure to 80 umol/L IAA plus 1.2 μg/mL HRP for 72 h, the apoptosis rate increased to 72.5per thousand, which was nine times that of control. Content of MDA and activity of SOD increased respectively after treatment compared to control. Meanwhile, IAA/HRP stimulated the formation of free radicals. CONCLUSION: The combination of IAA and HRP can inhibit the growth of human pancreatic cancer BXPC-3 cells in vitro by inducing apoptosis.
机译：AM：探讨吲哚-3-乙酸（IAA）结合辣根过氧化物酶（HRP）诱导人胰腺癌BXPC-3细胞凋亡的潜在机制。方法：将人胰腺癌衍生的BXPC-3细胞在不同时间暴露于40或80μmol/ L IAA和1.2μg/ mL HRP。然后，MTT法用于检测细胞增殖。进行流式细胞仪分析细胞周期。末端脱氧核苷酸转移酶介导的dUTP缺口末端标记法用于检测细胞凋亡。通过共聚焦显微镜测量2，7-二氯荧光素二乙酸酯的摄取以确定自由基。通过生化方法测量丙二醛（MDA）含量和超氧化物歧化酶（SOD）活性。结果：IAA / HRP以剂量和时间依赖性方式开始抑制BXPC-3细胞的生长。流式细胞仪显示处理48小时的细胞停滞在G_1 / G_0。暴露于80 umol / L IAA加1.2μg/ mL HRP 72 h后，细胞凋亡率增加至72.5 /千，是对照组的9倍。与对照相比，处理后MDA含量和SOD活性分别增加。同时，IAA / HRP刺激了自由基的形成。结论：IAA和HRP联合应用可通过诱导细胞凋亡来抑制人胰腺癌BXPC-3细胞的生长。

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