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首页> 外文期刊>World Journal of Gastroenterology >Inhibitory effects of apigenin on the growth of gastric carcinoma SGC-7901 cells
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Inhibitory effects of apigenin on the growth of gastric carcinoma SGC-7901 cells

机译：芹菜素对胃癌SGC-7901细胞生长的抑制作用

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摘要

AIM: To explore the growth inhibition and apoptosis-inducing effect of apigenin on human gastric carcinoma SGC-7901 cells. METHODS: The effects of apigenin on the growth, clone formation and proliferation of human gastric carcinoma SGC-7901 cells were observed by MTT, clone-forming assay, and morphological observation. Fluorescent staining and flow cytometry analysis were used to detect apoptosis of cells. RESULTS: Apigenin obviously inhibited the growth, clone formation and proliferation of SGC-7901 cells in a dose-dependent manner. Inhibition of growth was observed on d 1 at the concentration of 80 μmol/L, while after 4 d, the inhibition rate (IR) was 90%. The growth IRs at the concentration of 20, 40, and 80 μmol/L were 38%, 71%, and 99% respectively on the 7th d. After the cells were treated with apigenin for 48 h, the number of done-forming in control, 20, 40, and 80 μmol/L groups was 217±16.9, 170±11.1 (P < 0.05), 98±11.1 (P < 0.05), and 25±3.5 (P < 0.05) respectively. Typical morphological changes of apoptosis was found by fluorescent staining. The cell nuclei had lost its smooth boundaries, chromatin was condensed, and cell nuclei were broken. Flow cytometry detected typical apoptosis peak. After the cells were treated with apigenin for 48 h, the apoptosis rates were 5.76%, 19.17%, and 29.30% respectively in 20, 40, and 80 μmol/L groups. CONCLUSION: Apigenin shows obvious inhibition on the growth and clone formation of SGC-7901 cells by inducing apoptosis.
机译：目的：探讨芹菜素对人胃癌SGC-7901细胞的生长抑制和诱导凋亡的作用。方法：采用MTT法，克隆形成法和形态学观察方法观察芹菜素对人胃癌SGC-7901细胞生长，克隆形成和增殖的影响。荧光染色和流式细胞仪分析用于检测细胞凋亡。结果：芹菜素明显抑制SGC-7901细胞的生长，克隆形成和增殖。在d 1浓度为80μmol/ L时观察到生长抑制，而在4 d后，抑制率（IR）为90％。在第7天，浓度为20、40和80μmol/ L的生长IR分别为38％，71％和99％。用芹菜素处理细胞48 h后，对照组，20、40和80μmol/ L组的完成形成数量分别为217±16.9、170±11.1（P <0.05），98±11.1（P < 0.05）和25±3.5（P <0.05）。通过荧光染色发现凋亡的典型形态学变化。细胞核失去了平滑的边界，染色质浓缩，细胞核破裂。流式细胞仪检测到典型的凋亡峰。用芹菜素处理细胞48 h后，在20、40和80μmol/ L组中凋亡率分别为5.76％，19.17％和29.30％。结论：芹菜素通过诱导细胞凋亡对SGC-7901细胞的生长和克隆形成有明显的抑制作用。

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