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首页> 外文期刊>World Journal of Gastroenterology >Functional expression of a proliferation-related ligand in hepatocellular carcinoma and its implications for neovascularization.
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Functional expression of a proliferation-related ligand in hepatocellular carcinoma and its implications for neovascularization.

机译:肝细胞癌中增殖相关配体的功能性表达及其对新血管形成的影响。

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AIM: To detect the expression of a proliferation-related ligand on human hepatocellular carcinoma (HCC) cell lines (SK-Hep1, HLE and HepG2) and in culture medium. METHODS: APRIL expression was analyzed by Western blotting in HCC cell lines. Effects of APRIL to cell count and angiogenesis were analyzed, too. RESULTS: Recombinant human APRIL (rhAPRIL) increased cell viability of HepG2 cells and, in HUVEC, rhAPRIL provided slight tolerance to cell death from serum starvation. Soluble APRIL (sAPRIL) from HLE cells increased after serum starvation, but did not change in SK-Hep1 or HepG2 cells. These cells showed down-regulation of VEGF after incubation with anti-APRIL antibody. Furthermore, culture medium from the HCC cells treated with anti-APRIL antibody treatment inhibited tube formation of HUVECs. CONCLUSION: Functional expression of APRIL might contribute to neovascularization via an upregulation of VEGF in HCC.
机译:目的:检测人肝细胞癌(HCC)细胞系(SK-Hep1,HLE和HepG2)和培养基中增殖相关配体的表达。方法:通过Western blotting分析肝癌细胞株APRIL的表达。还分析了APRIL对细胞计数和血管生成的影响。结果:重组人APRIL(rhAPRIL)增加了HepG2细胞的细胞活力,在HUVEC中,rhAPRIL对因血清饥饿导致的细胞死亡提供了轻微的耐受性。血清饥饿后,来自HLE细胞的可溶性APRIL(sAPRIL)增加,但在SK-Hep1或HepG2细胞中没有变化。与抗APRIL抗体孵育后,这些细胞显示出VEGF的下调。此外,用抗APRIL抗体处理的HCC细胞的培养基抑制了HUVEC的管形成。结论:APRIL的功能性表达可能通过肝癌中VEGF的上调而促进新生血管形成。

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