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首页> 外文期刊>World Journal of Gastroenterology >Dynamic expression of apoptosis-related genes during development of laboratory hepatocellular carcinoma and its relation to apoptosis.
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Dynamic expression of apoptosis-related genes during development of laboratory hepatocellular carcinoma and its relation to apoptosis.

机译：实验性肝细胞癌发展过程中凋亡相关基因的动态表达及其与凋亡的关系。

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AIM: To explore the expression of p53, bcl-2, bax, survivin and the cell apoptosis during the development of tree shrew hepatocellular carcinoma (HCC), the relationship between expression of these genes, its impact on HCC development, and its relation to cell apoptosis. METHODS: Tree shrew HCC was induced with aflatoxin B1 (AFB1), and regular biopsy of liver tissues was carried out and the biopsy tissues were collected during cancer inducement. Liver biopsy tissue and HCC tissue were collected from 35 pre-cancerous experimental animals at wk 30 and 60 and at the 30(th)-, 60(th)-, and 90(th)-wk. Liver biopsy tissues were collected from 13 blank control animals at wk 30, 60, and 90. Expression of p53, bcl-2, bax, and survivin at each stage was examined by immunohistochemistry method. Apoptotic cells were detected in situ by the terminal deoxynucleotidyl transferase-mediated nick end labeling (TUNEL) technique. RESULTS: The apoptosis rate of normal hepatic cells was extremely low, whereas it increased during the formation of HCC. Expression of the apoptosis-related genes p53, bcl-2, bax, and survivin during the formation of HCC presented an increasing tendency. Expression of p53 did not noticeably relate to that of bcl-2, bax, and survivin, whereas expression of bcl-2 and bax was closely related. In HCC, p53 did not present a distinct relation to cell apoptosis, whereas its high level expression was probably related to liver cell proliferation. Survivin negatively correlated apoptosis index, and its overexpression could inhibit cell apoptosis. CONCLUSION: Apoptosis-related genes p53, bcl-2, bax, and survivin are all related to the occurrence of HCC. The anti-apoptosis effect of bcl-2 is influenced by bax, and ratio bcl/bax reflects more correctly the extent of cell apoptosis.
机译：目的：探讨树sh肝细胞癌（HCC）发育过程中p53，bcl-2，bax，survivin的表达及细胞凋亡，这些基因的表达之间的关系，其对肝癌发生的影响及其与肝癌的关系。细胞凋亡。方法：用黄曲霉毒素B1（AFB1）诱导树HHCC，并在癌症诱导过程中定期对肝组织进行活检，并收集活检组织。从第30周和第60周以及第30、60、90周的35例癌前实验动物中收集肝活检组织和HCC组织。从第30、60和90周的13只空白对照动物收集肝活检组织。通过免疫组织化学方法检查每个阶段的p53，bcl-2，bax和survivin的表达。通过末端脱氧核苷酸转移酶介导的切口末端标记（TUNEL）技术原位检测凋亡细胞。结果：正常肝细胞的凋亡率极低，而在肝癌形成过程中却增加。肝癌形成过程中凋亡相关基因p53，bcl-2，bax和survivin的表达呈上升趋势。 p53的表达与bcl-2，bax和survivin的表达无关，而bcl-2和bax的表达则密切相关。在HCC中，p53与细胞凋亡没有明显关系，而其高水平表达可能与肝细胞增殖有关。 Survivin与细胞凋亡指数呈负相关，其过表达可能抑制细胞凋亡。结论：凋亡相关基因p53，bcl-2，bax和survivin均与肝癌的发生有关。 bcl-2的抗凋亡作用受bax影响，bcl / bax比值更正确地反映了细胞凋亡的程度。

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