...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>World Journal of Gastroenterology >Synergistic effect of bromocriptine and tumor necrosis factor-alpha on reversing hepatocellular carcinoma multidrug resistance in nude mouse MDR1 model of liver neoplasm.
【24h】

Synergistic effect of bromocriptine and tumor necrosis factor-alpha on reversing hepatocellular carcinoma multidrug resistance in nude mouse MDR1 model of liver neoplasm.

机译：溴隐亭和肿瘤坏死因子-α在肝癌裸鼠MDR1模型中逆转肝细胞癌多药耐药性的协同作用。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

AIM: To investigate the effect of bromocriptine (BCT) and tumor necrosis factor-alpha (TNF-alpha) on hepatocellular carcinoma (HCC) multidrug resistance (MDR) in nude mouse MDR model of liver neoplasm. METHODS: Human hepatocarcinoma cell line HepG(2), drug resistant hepatocarcinoma cell line HepG(2)/adriamycin (ADM) and hepatocarcinoma cell line transfected with TNF-alpha gene HepG(2)/ADM/TNF were injected into the liver of nude mice via orthotopic implantation and MDR model of liver neoplasm in vivo was established (HepG(2), ADM, TNF, BCT groups). Among these groups, BCT group and TNF group were treated with BCT through gastric canal. Each group was divided into control group and chemotherapy group. Size and weight of the tumor were measured. Furthermore, tumor histological character and growth of the nude mice were observed and their chemosensitivity was tested. MDR-associated genes and proteins (MRP, LRP) of implanted tumors were detected by immunohistochemistry, reverse transcriptase polymerase chain reaction, and apoptosis rate of hepatocarcinoma cells was detected by TUNEL assay. RESULTS: The nude mouse model of each cell line was inoculated successfully. The tumor growth rate and weight were significantly different among groups. After chemotherapy, abdominal cavity tumor growth inhibition rate was higher in BCT group (67%) compared to ADM and TNF groups, and similar to HepG(2) group (54%). MDR1 and LRPmRNA could be detected in all groups, but TNF-alpha was detected only in TNF and BCT groups. Furthermore, MDR1 and LRP protein expression of tumors in TNF and BCT groups was low similar to HepG(2) group. The apoptosis rate of hepatocarcinoma cells was much higher in BCT group than in other groups with TUNEL assay. CONCLUSION: BCT and TNF-alpha can reverse HCC MDR in nude mouse MDR1 model of liver neoplasm.
机译：目的：探讨溴隐亭（BCT）和肿瘤坏死因子-α（TNF-α）对肝癌裸鼠MDR模型中肝细胞癌（HCC）多药耐药（MDR）的影响。方法：将人肝癌细胞系HepG（2），耐药性肝癌细胞系HepG（2）/阿霉素（ADM）和转染TNF-α基因HepG（2）/ ADM / TNF的肝癌细胞系注入裸鼠肝脏通过原位植入和体内肝肿瘤MDR模型建立小鼠（HepG（2），ADM，TNF，BCT组）。在这些组中，BCT组和TNF组通过胃管进行BCT治疗。每组分为对照组和化疗组。测量肿瘤的大小和重量。此外，观察裸鼠的肿瘤组织学特征和生长，并测试其化学敏感性。免疫组化，逆转录酶聚合酶链反应检测移植瘤的MDR相关基因和蛋白（MRP，LRP），TUNEL法检测肝癌细胞的凋亡率。结果：成功接种各细胞系裸鼠模型。各组之间的肿瘤生长速度和重量显着不同。化疗后，与ADM和TNF组相比，BCT组的腹腔肿瘤生长抑制率更高（67％），与HepG（2）组相似（54％）。在所有组中均可检测到MDR1和LRPmRNA，但仅在TNF和BCT组中检测到TNF-α。此外，TNF和BCT组中肿瘤的MDR1和LRP蛋白表达与HepG（2）组相似。 TUNEL法检测BCT组肝癌细胞凋亡率明显高于其他各组。结论：BCT和TNF-α可以逆转裸鼠肝癌MDR1模型中的HCC MDR。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号