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首页> 外文期刊>World Journal of Gastroenterology >Dendritic cells pulsed with hsp70-peptide complexes derived from human hepatocellular carcinoma induce specific anti-tumor immune responses.
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Dendritic cells pulsed with hsp70-peptide complexes derived from human hepatocellular carcinoma induce specific anti-tumor immune responses.

机译：用衍生自人肝细胞癌的hsp70-肽复合物脉冲的树突状细胞诱导特异性抗肿瘤免疫反应。

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摘要

AIM: To investigate the anti-tumor effect of dendritic cells (DCs) pulsed with hsp70-peptide complexes derived from human hepatocellular carcinoma (HCC) cells on human T cells. METHODS: Hsp70-peptide complexes were purified from human HCC cells with column chromatography using ADP-agarose and DEAE-Sepharose. DCs were derived from peripheral blood mononuclear cells of healthy donors in the presence of human GM-CSF and IL-4. The anti-tumor effect of DCs pulsed with hsp70-peptide complexes on human T-cell was assayed by CTL and enzyme-linked immunospot (ELISPOT) tests. RESULTS: Hsp70-peptide complexes derived from human HCC cells activated phenotypic and functional maturation of DCs. The matured DCs stimulated a high level of autologous T-cell proliferation and type I cytokine secretion, and induced HCC-specific cytotoxic T lymphocytes (CTLs), which specifically killed HCC cells by a MHC class I restricted mechanism. CONCLUSION: Hsp70-peptide complexes derived from human HCC cells can serve as a potent tumor antigen source for pulsing DCs, the pulsed DCs are very effective in activating specific T-cell responses against HCC cells.
机译：目的：研究用人肝细胞癌（HCC）细胞衍生的hsp70-肽复合物脉冲的树突状细胞（DC）对人T细胞的抗肿瘤作用。方法：采用ADP-琼脂糖和DEAE-Sepharose柱层析法从人HCC细胞中纯化Hsp70-肽复合物。 DCs来自存在人类GM-CSF和IL-4的健康供体的外周血单核细胞。通过CTL和酶联免疫斑点（ELISPOT）测试来分析用hsp70-肽复合物脉冲的DC对人T细胞的抗肿瘤作用。结果：从人类肝细胞的Hsp70肽复合物激活DC的表型和功能成熟。成熟的DC刺激了高水平的自体T细胞增殖和I型细胞因子分泌，并诱导了HCC特异性细胞毒性T淋巴细胞（CTL），后者通过MHC I类限制性机制特异性杀死了HCC细胞。结论：人HCC细胞衍生的Hsp70-肽复合物可作为脉冲DC的有效肿瘤抗原来源，脉冲DC在激活针对HCC细胞的特异性T细胞应答中非常有效。

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