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首页> 外文期刊>World Journal of Gastroenterology >Value of endoscopic methylene blue and Lugol's iodine double staining and detection of GST-Pi and telomerase in the early diagnosis of esophageal carcinoma.
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Value of endoscopic methylene blue and Lugol's iodine double staining and detection of GST-Pi and telomerase in the early diagnosis of esophageal carcinoma.

机译：内窥镜亚甲蓝和Lugol's碘双重染色及GST-Pi和端粒酶检测在食管癌早期诊断中的价值。

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AIM: To explore the expressions of GST-Pi and telomerase activity in esophageal carcinoma and premalignant lesions and to investigate the value of endoscopic methylene blue (MB) and Lugol's iodine double staining. METHODS: Seventy-two patients with esophagopathy were sprayed endoscopically with MB and Lugol's iodine in proper order and the areas stained blue and brown, and the area between the blue and brown stains were obtained. Depending on the pattern of mucosal staining, biopsy specimen was obtained. GST-Pi and telomerase activity in specimens were examined by immunohis-tochemistry and PCR-based silver staining telomeric repeat amplification protocol, respectively. RESULTS: After MB and Lugol's iodine staining, the area between both the colors was obtained in 64 of the 72 patients and the areas were stained blue and brown in all of the 72 patients. Association test of two simultaneous ordinal categorical data showed a correlation between the esophageal mucosal staining and the esophageal histology (P<0.005). The expression of GST-Pi and telomerase activity in esophageal carcinoma and premalignant lesions increased. The expression of GST-Pi and telomerase activity in dysplasia and carcinoma was significantly higher than that in normal epithelium (P<0.005). The expression in hyperplasia was slightly higher than that in normal epithelium. With the lesions progressing from low- to moderate- to high-grade dysplasia, the positive rate increased (P<0.025). Expression of GST-Pi was correlated with that of telomerase activity in dysplasia and carcinoma (phis = 0.4831, P<0.005; phis = 0.3031, P<0.025, respectively); but there was no correlation between them in normal epithelium and hyperplasia. CONCLUSION: The expression of GST-Pi and telomerase may be an early event in the carcinogenesis of esophagus. They may play an induced and synergistic role with each other in the carcinogenesis of esophagus. Endoscopic MB and Lugol's iodine double staining and detection of GST-Pi and telomerase activity may contribute to the early diagnosis of esophageal carcinoma.
机译：目的：探讨GST-Pi和端粒酶活性在食管癌及癌前病变中的表达，探讨内镜下亚甲蓝（MB）和卢戈尔碘双重染色的价值。方法：对72例食管病患者进行内镜下MB和Lugol's碘的适当喷雾，染成蓝色和棕色，并获得蓝色和棕色之间的区域。根据粘膜染色的方式，获得活检标本。分别通过免疫组化和基于PCR的银染端粒重复扩增方案检查了标本中的GST-Pi和端粒酶活性。结果：MB和Lugol氏碘染色后，在72名患者中有64名获得了两种颜色之间的区域，在所有72名患者中均将其染成了蓝色和棕色。两项同时有序分类数据的关联测试显示，食管粘膜染色与食管组织学之间存在相关性（P <0.005）。食管癌和癌前病变中GST-Pi的表达和端粒酶活性增加。非典型增生和癌组织中GST-Pi的表达和端粒酶活性明显高于正常上皮（P <0.005）。增生中的表达略高于正常上皮中的表达。随着病变从低度发展到中度至高度不典型增生，阳性率增加（P <0.025）。在异型增生和癌中，GST-Pi的表达与端粒酶活性相关（phis = 0.4831，P <0.005； phis = 0.3031，P <0.025）；但正常上皮和增生之间没有相关性。结论：GST-Pi和端粒酶的表达可能是食管癌变的早期事件。它们在食道的癌变过程中可能彼此起诱导和协同作用。内镜下MB和Lugol's碘双重染色以及GST-Pi和端粒酶活性的检测可能有助于食管癌的早期诊断。

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