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首页> 外文期刊>World Journal of Gastroenterology >Alterations of intestinal immune function and regulatory effects of L-arginine in experimental severe acute pancreatitis rats.
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Alterations of intestinal immune function and regulatory effects of L-arginine in experimental severe acute pancreatitis rats.

机译：实验性重症急性胰腺炎大鼠肠道免疫功能的改变和L-精氨酸的调节作用。

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摘要

AIM: To discuss the changes of intestinal mucosal immune function in rats with experimental severe acute pancreatitis (SAP) and the regulatory effect of L-arginine. METHODS: Male adult Wistar rats were randomly divided into pancreatitis group, sham-operation group, and L-arginine treatment group. Animals were killed at 24, 48, and 72 h after SAP models were developed and specimens were harvested. Endotoxin concentration in portal vein was determined by limulus endotoxin analysis kit. CD3+, CD4+, CD8+ T lymphocytes in intestinal mucosal lamina propria were examined by immunohistochemistry. Secretory immunoglobulin A (SIgA) in cecum feces was examined by radioimmunoassay. RESULTS: Compared to the control group, plasma endotoxin concentration in the portal vein increased, percentage of CD3+ and CD4+ T lymphocyte subsets in the end of intestinal mucosal lamina propria reduced significantly, CD4+/CD8+ ratio decreased, and SIgA concentrations in cecum feces reduced at 24, 48, and 72 h after SAP developed. Compared to SAP group, the L-arginine treatment group had a lower level of plasma endotoxin concentration in the portal vein, a higher CD3+ and CD4+ T lymphocyte percentage in the end of intestinal mucosal lamina propria, an increased ratio of CD4+/CD8+ and a higher SIgA concentration in cecum feces. CONCLUSION: Intestinal immune suppression occurs in the early stage of SAP rats, which may be the main reason for bacterial and endotoxin translocation. L-arginine can improve the intestinal immunity and reduce bacterial and endotoxin translocation in SAP rats.
机译：目的：探讨实验性重症急性胰腺炎（SAP）大鼠肠道粘膜免疫功能的变化及L-精氨酸的调节作用。方法：雄性成年Wistar大鼠随机分为胰腺炎组，假手术组和L-精氨酸治疗组。建立SAP模型并收集标本后，在24、48和72 h处死动物。用内毒素分析试剂盒测定门静脉内毒素的浓度。通过免疫组织化学检查肠道粘膜固有层中的CD3 +，CD4 +，CD8 + T淋巴细胞。盲肠粪便中的分泌性免疫球蛋白A（SIgA）通过放射免疫法检查。结果：与对照组相比，肠粘膜固有层末端的血浆内毒素浓度增加，CD3 +和CD4 + T淋巴细胞亚群的百分比明显降低，CD4 + / CD8 +比降低，盲肠粪中SIgA浓度降低。 SAP开发后的24、48和72小时。与SAP组相比，L-精氨酸治疗组门静脉血浆内毒素浓度较低，肠黏膜固有层末端的CD3 +和CD4 + T淋巴细胞百分比较高，CD4 + / CD8 +比例增加，且盲肠粪便中SIgA浓度较高。结论：肠道免疫抑制发生在SAP大鼠的早期，可能是细菌和内毒素易位的主要原因。 L-精氨酸可以改善SAP大鼠的肠道免疫力并减少细菌和内毒素的转运。

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