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首页> 外文期刊>World Journal of Gastroenterology >Apoptosis of human gastric adenocarcinoma cells induced by β-ionone
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Apoptosis of human gastric adenocarcinoma cells induced by β-ionone

机译：β-紫罗兰酮诱导人胃腺癌细胞凋亡

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摘要

AIM: To investigate the effect of β-ionone on the growth and apoptosis of gastric adenocarcinoma cell line SGC-7901. METHODS: Using MTT, fluorescence dye (Hoechst-33258), transmission electron microscopy and the TUNEL assay, we examined growth and apoptosis of SGC-7901 cells treated with β-ionone at various concentrations (i.e. 25, 50, 100 and 200 μmol/L) for 24 h, 48 h. RESULTS: The growth of SGC-7901 cells was inhibited by p-ionone. Seven days after treatment with β-ionone at four concentrations, the inhibition rates were 12.04%, 30.59%, 78.25% and 94.15%, respectively. The IC_(50) value of β-ionone for SGC-7901 cells was estimated to be 89 μmol/L. The apoptotic morphology was demonstrated in SGC-7901 cells treated with β-ionone by Hoechst-33258 staining and electron microscopy. Apoptosis was also shown in β-ionone-treated SGC-7901 cells by the TUNEL assay. CONCLUSION: β-ionone can inhibit cell proliferation and induce apoptosis of SGC-7901 cells. However, the mechanism needs to be further investigated.
机译：目的：探讨β-紫罗兰酮对胃腺癌细胞SGC-7901生长和凋亡的影响。方法：使用MTT，荧光染料（Hoechst-33258），透射电子显微镜和TUNEL测定法，我们研究了在不同浓度（即25、50、100和200μmol/ ml的β-紫罗兰酮处理下的SGC-7901细胞的生长和凋亡）。 L）24小时，48小时。结果：p-紫罗兰酮抑制了SGC-7901细胞的生长。用四种浓度的β-紫罗兰酮处理7天后，抑制率分别为12.04％，30.59％，78.25％和94.15％。对于SGC-7901细胞，β-紫罗兰酮的IC_（50）值估计为89μmol/ L。通过Hoechst-33258染色和电子显微镜观察，在用β-紫罗兰酮处理的SGC-7901细胞中显示了凋亡形态。通过TUNEL分析，在β-紫罗兰酮处理的SGC-7901细胞中也显示出凋亡。结论：β-紫罗兰酮可以抑制SGC-7901细胞的增殖并诱导其凋亡。但是，该机制需要进一步研究。

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