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首页> 外文期刊>World Journal of Gastroenterology >Downregulation of retinoic acid receptor-β_2 expression is linked to aberrant methylation in esophageal squamous cell carcinoma cell lines
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Downregulation of retinoic acid receptor-β_2 expression is linked to aberrant methylation in esophageal squamous cell carcinoma cell lines

机译：维甲酸受体β_2表达下调与食管鳞癌细胞株甲基化异常有关

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摘要

AIM: To study the role of hypermethylation in the loss of retinoic acid receptor β_2(RARβ_2) in esophageal squamous cell carcinoma (ESCC). METHODS: The role of hypermethylation in RARβ_2 gene silencing in 6 ESCC cell lines was determined by methylation-specific PCR (MSP), and its methylation status was compared with RARβ_2 mRNA expression by RT-PCR. The MSP results were confirmed by bisulfite sequencing of RARβ_2 promoter regions. RESULTS: Methylation was detected in 4 of the 6 cell lines, and the expression of RARβ_2 was markedly downregulated in 3 of the 4 methylated cell lines. The expression of RARβ_2 was restored in one RARβ_2-downregulated cell line with the partial demethylation of promoter region of RARβ_2 after 5-aza-2'-deoxycytidine (5-aza-dc) treatment. CONCLUSION: The methylation of the 5' region may play an important role in the downregulation of RARβ_2 in some ESCC cell lines, suggesting that multiple mechanisms contribute to the loss of RARβ_2 expression in ESCC cell lines. This study may have clinical applications for treatment and prevention of ESCC.
机译：目的：研究高甲基化在食管鳞状细胞癌（ESCC）视黄酸受体β_2（RARβ_2）丢失中的作用。方法：采用甲基化特异性PCR（MSP）检测高甲基化在6种ESCC细胞RARβ_2基因沉默中的作用，并通过RT-PCR比较其甲基化状态与RARβ_2mRNA的表达。通过RARβ_2启动子区域的亚硫酸氢盐测序证实了MSP结果。结果：在6个细胞系中有4个检测到甲基化，在4个甲基化细胞系中的3个中RARβ_2的表达明显下调。在5-氮杂-2'-脱氧胞苷（5-氮杂-dc）处理后，在一个RARβ_2下调的细胞系中，RARβ_2的启动子区域被部分去甲基化，从而恢复了RARβ_2的表达。结论：5'区域甲基化可能在某些ESCC细胞系中RARβ_2的下调中起重要作用，提示多种机制可能导致ESCC细胞系RARβ_2表达的丧失。这项研究可能具有治疗和预防ESCC的临床应用。

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