• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>World Journal of Gastroenterology >Transcriptionai regulation of human α1(I) procollagen gene in dermal fibroblasts
【24h】

Transcriptionai regulation of human α1(I) procollagen gene in dermal fibroblasts

机译：皮肤成纤维细胞中人α1（I）前胶原基因的转录调控

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

AIM: To clarify the fractional activity of promoters from human α1(I) procollagen gene, the interaction between cis-elements and consensus DNA-binding proteins responsible for high promoter activity, and the potential application of promoter competitors as well as cytokines for antifibrogenesis. METHODS: Sequence between 2 483 bp upstream of the start of transcription and 42 bp downstream of this site was investigated with serial 5′-deletion. The 5′-deleted promoters recombined with chloramphenicol acetyltransferase (CAT) as reporter gene were transiently transfected to human dermal fibroblasts. Electrophoretic mobility shift assay was performed to show the DNA-protein binding capacity of the promoter sequence. Cytokines including tumor necrosis factor α (TNFα) and interferons (INFs) were added to the culture medium of transiently transfected fibroblasts. Competitor DNA for the binding sites of Sp-1, Ap-1 and NF-1 was individually cotransfected transiently in order to block the promoter-driven CAT expression. RESULTS: Sequences of -2 483 to +42 bp and -268 to +42 bp of human α1(I) procollagen gene had high activity as promoters. Binding sites for Ap-1 and Sp-1 were among the cis-regulatory elements recognizing consensus transcription factors responsible for basal promoter activity of sequence -268 to +42 bp. TNFα, IFNα, IFNβ showed inhibitory effects on sequence -2 483 to +42 bp as promoter with activities 43%, 62% and 60% of control respectively. Transfection of the promoter competitors could reverse the promoter activity of -268 to +42 bp 40-60%. CONCLUSION: Sequences of -2 483 to +42 bp recombined with reporter gene provide an ideal construction for transcriptionai study of α1(I) procollagen gene. The anti-collagen capacity of TNFα and IFNs is associated with their transcriptionai regulation. Ap-1 and Sp-1 mediate the basal transcriptionai activation of human α1(I) procollagen gene in dermal fibroblasts. Competitors for highly active promoters might be a novel potential candidate in fibrotic blockade.
机译：目的：为了阐明人类α1（I）前胶原基因启动子的部分活性，负责高启动子活性的顺式元件与共有DNA结合蛋白之间的相互作用，以及启动子竞争者以及抗纤化细胞因子的潜在应用。方法：通过序列5'缺失研究了转录起始上游2 483 bp与该位点下游42 bp之间的序列。与氯霉素乙酰转移酶（CAT）作为报告基因重组的5'缺失启动子被瞬时转染到人类皮肤成纤维细胞中。进行电泳迁移率变动分析以显示启动子序列的DNA-蛋白质结合能力。将包括肿瘤坏死因子α（TNFα）和干扰素（INFs）的细胞因子添加到瞬时转染的成纤维细胞的培养基中。 Sp-1，Ap-1和NF-1结合位点的竞争者DNA分别被瞬时共转染，以阻断启动子驱动的CAT表达。结果：人α1（I）前胶原基因的-2 483至+42 bp和-268至+42 bp序列具有很高的启动子活性。 Ap-1和Sp-1的结合位点是顺式调节元件，可识别负责序列启动子活性的-268至+42 bp的共有转录因子。 TNFα，IFNα，IFNβ对作为启动子的序列-2483至+ 42bp显示抑制作用，活性分别为对照的43％，62％和60％。启动子竞争者的转染可将-268的启动子活性逆转至+42 bp 40-60％。结论：与报道基因重组的-2 483至+42 bp序列为α1（I）原胶原基因的转录研究提供了理想的构建。 TNFα和IFN的抗胶原能力与其转录调控有关。 Ap-1和Sp-1介导皮肤成纤维细胞中人α1（I）前胶原基因的基础转录激活。高活性启动子的竞争者可能是纤维化阻断的新型潜在候选者。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号