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首页> 外文期刊>World Journal of Gastroenterology >Constitutive activation of Stat3 signaling pathway in human coIorectaI carcinoma
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Constitutive activation of Stat3 signaling pathway in human coIorectaI carcinoma

机译：结肠直肠癌Stat3信号通路的组成性激活

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AIM: Signal transducers and activators of transcription (STATs) are a family of transcription factors activated in response to cytokines and growth factors. Constitutive activation of Stat3 has been observed in a growing number of tumor-derived cell lines, as well as tumor specimens from human cancers. The purpose of this study was to investigate the expression of p-Stat3, activated form of Stat3, and its downstream mediators including cyclin D1 and Bcl-X_L in colorectal carcinoma (CRC), and to explore the possible mechanism of Stat3 signaling pathway in the tumorigenesis of colorectal carcinoma. METHODS: Tissue samples from 45 patients of primary colorectal carcinoma were selected for studying Stat3 signaling pathway protein expression. Western blot analysis was used to measure the expression of p-Stat3, cyclin D1, and Bcl-X_L proteins in colorectal carcinomas. Furthermore, the expression patterns of these proteins were analyzed for their distribution at the cellular level by immunohistochemical staining of the tissues. RESULTS: Protein levels of p-Stat3, cyclin D1, and Bcl-X_L were increased in colorectal carcinomas compared with adjacent normal mucosae (P<0.05). Elevated levels of p-Stat3 were correlated with the nodal metastasis and the stage (P<0.05). Overexpression of cyclin D1 was associated with the nodal metastasis (P<0.05). There was also a significant correlation between the expressions of p-Stat3 and cyclin D1 (r=0.382, P<0.05). CONCLUSION: Constitutive activation of Stat3 may play an important role in the tumorigenesis of colorectal carcinoma, and the detailed mechanism of Stat3 signaling pathway in CRC deserves further investigation.
机译：目的：信号转导子和转录激活子（STATs）是响应细胞因子和生长因子而激活的转录因子家族。在越来越多的肿瘤衍生细胞系以及人类癌症的肿瘤标本中都观察到Stat3的组成性激活。这项研究的目的是调查p-Stat3，Stat3的活化形式及其下游介质（包括细胞周期蛋白D1和Bcl-X_L）在结直肠癌（CRC）中的表达，并探讨Stat3信号通路在大肠癌中的可能机制。大肠癌的肿瘤发生。方法：选择45例原发性大肠癌患者的组织样本用于研究Stat3信号通路蛋白的表达。 Western印迹分析用于测量大肠癌中p-Stat3，cyclin D1和Bcl-X_L蛋白的表达。此外，通过组织的免疫组织化学染色分析了这些蛋白质的表达模式在细胞水平上的分布。结果：与邻近正常黏膜相比，结直肠癌中p-Stat3，cyclin D1和Bcl-X_L蛋白水平升高（P <0.05）。 p-Stat3水平升高与淋巴结转移和分期相关（P <0.05）。细胞周期蛋白D1过表达与淋巴结转移有关（P <0.05）。 p-Stat3和细胞周期蛋白D1的表达之间也存在显着相关性（r = 0.382，P <0.05）。结论：Stat3的组成性激活可能在结直肠癌的发生中起重要作用，Stat3信号转导通路在CRC中的详细机制值得进一步研究。

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