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首页> 外文期刊>World Journal of Gastroenterology >Management of carbon tetrachloride-induced acute liver injury in rats by syngeneic hepatocyte transplantation in spleen and peritoneal cavity
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Management of carbon tetrachloride-induced acute liver injury in rats by syngeneic hepatocyte transplantation in spleen and peritoneal cavity

机译：脾脏和腹膜腔内同种肝细胞移植治疗四氯化碳诱导的大鼠急性肝损伤

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AIM: Acute hepatitis may seldom have a fulminant course. In the treatment of this medical emergency, potential liver support measure must provide immediate and sufficient assistance to the hepatic function. The goal of our study was to study the adequacy of hepatocyte transplantation (HCTx) in two different anatomical sites, splenic parenchyma and peritoneal cavity, in a rat model of reversible acute hepatitis induced by carbon tetrachloride (CCI_4). METHODS: After CCI_4 intoxication, 84 male Wistar rats used as recipients were divided in to four experimental groups accordingly to their treatment: Group A (n=24): intrasplenic transplantation of 10x10~6 isolated hepatocytes, Group B (n=24): intraperitoneal transplantation of 20x10~6 isolated hepatocytes attached on plastic microcarriers, Group C (n=18): intrasplenic injection of 1 mL normal saline (sham-operated controls), Group D (n=18): intraperitoneal injection of 2.5 mL normal saline (sham-operated controls). Survival, liver function tests (LFT) and histology were studied in all four groups, on d 2, 5 and 10 post-HCTx. RESULTS: The ten-day survival (and mean survival) in the 4 groups was 72.2% (8.1+-3.1), 33.3% (5.4+-3.4), 0% (3.1+-1.3) and 33.3% (5.4+-3.6) in groups A, B, C, D, respectively (P_(AB)<0.05, P_(AC)<0.05, P_(BD)=NS). In the final survivors, LFT (except alkaline phosphatase) and hepatic histology returned to normal, independently of their previous therapy. Viable hepatocytes were identified within splenic parenchyma (in group A on d 2) and both in the native liver and the fatty tissue of abdominal wall (in group B on d 5). CONCLUSION: A significantly better survival of the intrasplenically transplanted animals has been demonstrated. Intraperitoneal hepatocytes failed to promptly engraft. A different timing between liver injury and intraperitoneal HCTx may give better results and merits further investigation.
机译：目的：急性肝炎很少会发作。在治疗这种紧急医疗情况时，潜在的肝脏支持措施必须立即为肝功能提供足够的帮助。我们的研究目标是研究在四氯化碳（CCI_4）诱导的可逆性急性肝炎大鼠模型中，脾实质和腹膜腔两个不同解剖部位的肝细胞移植（HCTx）的适当性。方法：将CCI_4中毒后，将84只雄性Wistar大鼠作为受体，根据其治疗情况分为四个实验组：A组（n = 24）：脾内移植10x10〜6个分离的肝细胞，B组（n = 24）：腹腔内移植附着在塑料微载体上的20x10〜6个分离的肝细胞，C组（n = 18）：脾内注射1 mL生理盐水（假手术对照组），D组（n = 18）：腹膜内注射2.5 mL生理盐水（假操作控件）。在HCTx术后第2、5和10天，对所有四个组的存活率，肝功能测试（LFT）和组织学进行了研究。结果：4组的10天生存率（和平均生存率）分别为72.2％（8.1 + -3.1），33.3％（5.4 + -3.4），0％（3.1 + -1.3）和33.3％（5.4 +- 3.6）分别在A，B，C，D组中（P_（AB）<0.05，P_（AC）<0.05，P_（BD）= NS）。在最终的幸存者中，LFT（碱性磷酸酶除外）和肝组织学均恢复到正常状态，与之前的治疗方法无关。在脾实质内（第2天的A组）以及天然肝和腹壁脂肪组织（第5天的B组）中都鉴定出了活的肝细胞。结论：已证实脾内移植动物的存活率明显提高。腹膜内肝细胞未能及时植入。肝损伤和腹膜内HCTx的时机不同可能会产生更好的结果，值得进一步研究。

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