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Function of oval cells in hepatocellular carcinoma in rats

机译：卵圆细胞在大鼠肝癌中的作用

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摘要

AIM: To study oval cells' pathological characteristics and relationship with the occurrence of hepatocellular carcinoma (HCC); to observe the form and structural characteristics of oval cells; to explore the expression characteristics of C-kit, PCNA mRNA and c-myc gene during the occurrence and development of HCC and the effect of ulinastatin (UTI) on C-kit and PCNA expression. METHODS: One hundred and twenty-five SD rats fed on 3,3'-diaminobenzidine (DAB) to construct HCC models were divided into control group, cancer-inducing group and UTI intervention group. In each group, rat liver samples were collected at weeks 2, 4, 6, 8,10,12,14, 16,18, 20, 22 and 24 respectively to study pathological distribution characteristics of oval cells in the process of carcinogenesis under optical microscope. Oval cells were separated by the methods of improved density gradient centrifugation and their structural characteristics were observed under optical microscope and electronic microscope respectively; the oval cells expressing C-kit and PCNA in the collected samples were observed by the methods of immunohistochemistry and image analysis and the expression of c-myc mRNA was also detected by reverse transcription polymerase chain reaction (RT-PCR). RESULTS: Oval cells proliferated firstly in the portal area then gradually migrated into hepatic parenchyma in the inducing group and intervention group. The oval cells distributed inside and outside the carcinoma nodes. The oval cells presented the characteristics of undifferentiated cells: a high ratio of nucleolus and cellular plasm and obvious nucleoli, rare organelle in plasm. Only a few mitochondria and endoplasmic reticulum and some villus-like apophysis on surface of cells could be seen. Cells stained with C-kit and PCNA antibody were mainly oval cells distributed in the portal area. The expression of c-myc mRNA increased with the progression of HCC. However, in the intervention group, UTI could retard its increase. CONCLUSION: Oval cells work throughout the development of HCC, and might play important roles in this process. c-myc gene may be a kind of promoter gene of HCC, and play a key role in hepatic injury and development of HCC. UTI could retard the occurrence of HCC.
机译：目的：研究卵圆细胞的病理特征及其与肝细胞癌（HCC）发生的关系。观察椭圆形细胞的形式和结构特征；目的探讨肝癌发生发展过程中C-kit，PCNA mRNA和c-myc基因的表达特征以及乌司他丁（UTI）对C-kit和PCNA表达的影响。方法：125只SD大鼠以3,3'-二氨基联苯胺（DAB）为食，建立HCC模型，分为对照组，诱导癌组和UTI干预组。每组分别在第2、4、6、8、10、12、14、16、18、20、22和24周收集大鼠肝脏样品，以研究在光致癌作用下卵圆形细胞的病理分布特征。显微镜。采用改进的密度梯度离心法分离卵细胞，分别在光学显微镜和电子显微镜下观察其结构特征。通过免疫组织化学和图像分析的方法，观察收集到的表达C-kit和PCNA的卵圆细胞，并通过逆转录聚合酶链反应（RT-PCR）检测c-myc mRNA的表达。结果：诱导组和干预组卵圆形细胞首先在门脉区域增殖，然后逐渐迁移至肝实质。椭圆形细胞分布在癌结的内部和外部。卵圆形细胞表现出未分化细胞的特征：核仁和细胞质比例高，核仁明显，质中稀少的细胞器。在细胞表面只能看到少量的线粒体和内质网以及一些绒毛状的隆突。 C-kit和PCNA抗体染色的细胞主要是分布在门静脉区域的卵圆形细胞。 c-myc mRNA的表达随肝癌的发展而增加。但是，在干预组中，UTI可能会阻止其增加。结论：卵圆形细胞在肝癌的整个发展过程中均起作用，并可能在此过程中发挥重要作用。 c-myc基因可能是肝癌的一种启动子基因，在肝损伤和肝癌的发生发展中起着关键作用。尿路感染可延缓肝癌的发生。

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  • 来源
    《World Journal of Gastroenterology》 |2004年第17期|p.2482-2487|共6页
  • 作者

    Chi-Hua Fang; Jia-Qing Gong; Wei Zhang;

  • 作者单位

    Department of Hepatobiliary Surgery, Zhujiang Hospital, First Military Medical University, Guangzhou 510282, Guangdong Province, China;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 消化系及腹部疾病;
  • 关键词

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