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首页> 外文期刊>World Journal of Gastroenterology >Down-modulation of heat shock protein 70 and up-modulation of Caspase-3 during schisandrin B-induced apoptosis in human hepatoma SMMC-7721 cells
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Down-modulation of heat shock protein 70 and up-modulation of Caspase-3 during schisandrin B-induced apoptosis in human hepatoma SMMC-7721 cells

机译：五味子蛋白B诱导人肝癌SMMC-7721细胞凋亡过程中热休克蛋白70的下调和Caspase-3的上调

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AIM: To investigate the effect of schisandrin B (Sch B) on proliferation and apoptosis of human hepatoma SMMC-7721 cells in vitro and regulation of Hsp70 and Caspases-3, 7, 9 expression by Sch B. METHODS: Human hepatoma cell line SMMC-7721 was cultured and treated with Sch B at various concentrations. Growth suppression was detected with MTT colorimetric assay. Cell apoptosis was confirmed by DNA ladder detection and flow cytometric analysis. The expression of Hsp70, Caspases-3, 7, 9 were analyzed by Western blot analysis. RESULTS: Sch B inhibited the growth of hepatoma SMMC-7721 cells in a dose-dependent manner, leading to a 50% decrease in cell number (LC50) value of 23.50 mg/L. Treatment with Sch B resulted in degradation of chromosomal DNA into small internucleosomal fragments, evidenced by the formation of a 180-200 bp DNA ladder on agarose gels. FCM analysis showed the peak areas of subdiploid at the increased concentration of Sch B. The results of Western bolt analysis showed that Hsp70 was down-regulated and Caspase-3 was up-regulated, while the activity of Caspases-7, -9 had no significant change. CONCLUSION: Sch B is able to inhibit the proliferation of human hepatoma SMMC-7721 cells and induce apoptosis, which goes through Caspase-3-dependent and Caspase-9-independent pathway accompanied with the down-regulation of Hsp70 protein expression at an early event.
机译：目的：探讨五味子素B（Sch B）对人肝癌SMMC-7721细胞增殖和凋亡的影响以及Sch B对Hsp70和Caspases-3、7、9表达的调控。方法：人肝癌细胞SMMC培养了-7721，并用各种浓度的Sch B处理。用MTT比色法检测生长抑制。 DNA梯形检测和流式细胞仪分析证实了细胞凋亡。通过蛋白质印迹分析来分析Hsp70，Caspases-3、7、9的表达。结果：Sch B以剂量依赖性方式抑制肝癌SMMC-7721细胞的生长，导致细胞数（LC50）值降低23.50 mg / L 50％。用Sch B处理可将染色体DNA降解为小的核小体片段，这可通过在琼脂糖凝胶上形成180-200 bp DNA阶梯来证明。 FCM分析显示在Sch B浓度增加时亚二倍体的峰面积。Western螺栓分析结果显示Hsp70下调而Caspase-3上调，而Caspases-7，-9的活性却没有重大变化。结论：Sch B能够抑制人肝癌细胞SMMC-7721的增殖并诱导细胞凋亡，其通过Caspase-3依赖性和Caspase-9非依赖性途径，并在早期事件中下调Hsp70蛋白表达。 。

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