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首页> 外文期刊>World Journal of Gastroenterology >Cloning and analysizing the up-regulated expression of transthyretin-related gene (LR_1) in rat liver regeneration following short interval successive partial hepatectomy
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Cloning and analysizing the up-regulated expression of transthyretin-related gene (LR_1) in rat liver regeneration following short interval successive partial hepatectomy

机译：短间隔连续部分肝切除术后大鼠肝脏再生中转甲状腺素相关基因（LR_1）表达上调的克隆与分析

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AIM: Cloning and analysizing the up-regulated expression of transthyretin-related gene following short interval successive partial hepatectomy (SISPH) to elucidate the mechanism of differentiation, division, dedifferentiation and redifferentiation in rat liver regeneration (LR). METHODS: Lobus external sinister and lobus centralis sinister, lobus centralis, lobus dexter, lobus candatus were removed one by one from rat liver at four different time points 4, 36, 36 and 36 hr (total time: 4 hr, 40 hr, 76 hr, 112 hr) respectively. Suppression subtractive hybridization (SSH) was carried out by using normal rat liver tissue as driver and the tissue following short interval successive partial hepatectomy (SISPH) as tester to construct a highly efficient forward-subtractive cDNA library. After screening, an interested EST fragment was selected by SSH and primers were designed according to the sequence of the EST to clone the full-length cDNA fragment using RACE (rapid amplification of cDNA end). Homologous detection was performed between the full-lenth cDNA and Genbank. RESULTS: Forward suppression subtractive hybridization (FSSH) library between 0 h and 112 h following SISPH was constructed and an up-regulated full-length cDNA (named LR1), which was related with the transthyretin gene, was cloned by rapid amplification of cDNA end. It was suggested that the gene is involved in the cellular dedifferentiation in LR following SISPH. CONCLUSION: Some genes were up-regulated in 112 h following SISPH in rat. LR_1 is one of these up-regulated expression genes which may play an important role in rat LR.
机译：目的：克隆和分析短间隔连续部分肝切除术（SISPH）后转甲状腺素相关基因的表达上调，以阐明大鼠肝再生（LR）分化，分裂，去分化和再分化的机制。方法：在四个不同的时间点4、36、36和36小时（分别为4小时，40小时，76小时）从大鼠肝脏中一一摘除Lobus外阴部和中央阴部，中央阴部，右脑右旋，加拿大阴唇小时，112小时）。以正常大鼠肝脏组织为驱动子，以短间隔连续部分肝切除术（SISPH）后的组织为检测子，进行抑制消减杂交（SSH），构建了高效的前减法cDNA文库。筛选后，通过SSH选择感兴趣的EST片段，并根据EST的序列设计引物，以使用RACE（cDNA末端的快速扩增）克隆全长cDNA片段。在全长cDNA和Genbank之间进行同源检测。结果：构建了SISPH后0 h至112 h之间的正向抑制消减杂交（FSSH）文库，并通过快速扩增cDNA末端克隆了与运甲状腺素蛋白基因相关的全长全长cDNA（命名为LR1）。 。提示该基因与SISPH后LR中的细胞去分化有关。结论：SISPH后112 h大鼠体内某些基因表达上调。 LR_1是这些上调表达基因之一，可能在大鼠LR中起重要作用。

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