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首页> 外文期刊>World Journal of Gastroenterology >Protective effects of transplanted and mobilized bone marrow stem cells on mice with severe acute pancreatitis
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Protective effects of transplanted and mobilized bone marrow stem cells on mice with severe acute pancreatitis

机译：移植和动员的骨髓干细胞对重症急性胰腺炎的保护作用

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摘要

AIM: To evaluate the protective effects of transplanted and mobilized bone marrow stem cells (BMSCs) on mice with severe acute pancreatitis (SAP) and to probe into their possible mechanisms. METHODS: A mouse model of SAP induced by intraperitoneal injections of L-arginine was employed in the present study. Two hundred female Balb/c mice weighing 18-22 g were randomly assigned into 4 groups. Group A was the stem cell mobilized group treated by injection of granulocyte-colony stimulating factor (G-CSF) into mice for 4 days at a dose of 40 μg·kg~(-1)·d~(-1) before induction of SAP. Group B was the group of BMSCs transplantation, in which the mice were given the isolated BMSCs via the tail vein 4 days prior to induction of SAP. Group C served as the model control and only SAP was induced. The mice without induction of SAP in group D acted as the normal control. At the time of animal sacrifice at 24,48 and 72 h after induction of SAP, blood samples were obtained and prepared to detect serum amylase, while the abdominal viscera were examined both grossly and microscopically for the observation of pathological changes. RESULTS: The mortality of mice in the model control, groups A and B was 34 %, 8 % and 10 % respectively within 72 h after induction of SAP. The serum level of amylase in the model control was significantly increased at all time points after induction of SAP as compared with that of the normal control (P<0.05-0.01). When the mice were pretreated with BMSCs' transplantation or G-CSF injection, their serum level of amylase was significantly reduced at 48 h and 72 h after induction of SAP in comparison with that of the model control (P<0.05-0.01). In accordance with these observations, both gross and microscopic examinations revealed that the pathological changes of SAP in mice pretreated with BMSCs transplantation or G-CSF injection were considerably attenuated as compared with those in the model control at all observed time points. CONCLUSION: Both transplanted allogenic and mobilized autologous BMSCs can protect mouse pancreas from severe damage in the process of SAP.
机译：目的：评估移植和动员的骨髓干细胞（BMSCs）对重症急性胰腺炎（SAP）小鼠的保护作用，并探讨其可能的机制。方法：本研究采用腹腔注射L-精氨酸诱导的SAP小鼠模型。将200只体重为18-22 g的Balb / c雌性小鼠随机分为4组。 A组为干细胞动员组，通过在诱导小鼠之前以40μg·kg〜（-1）·d〜（-1）的剂量向小鼠注射粒细胞集落刺激因子（G-CSF）4天。树液。 B组是BMSCs移植组，其中在诱导SAP之前4天通过尾静脉给小鼠分离的BMSCs。 C组作为模型对照，只有SAP被诱导。 D组中未诱导SAP的小鼠充当正常对照。在诱导SAP后24,48和72 h处死动物时，采集血样并准备检测血清淀粉酶，同时对腹部内脏进行肉眼和显微镜检查以观察病理变化。结果：模型对照组，A组和B组小鼠在诱导SAP后72小时内的死亡率分别为34％，8％和10％。与正常对照组相比，模型对照组在诱导SAP后的所有时间点血清淀粉酶水平均显着升高（P <0.05-0.01）。小鼠经骨髓间充质干细胞移植或G-CSF注射预处理后，与模型对照组相比，诱导SAP后48h和72h血清淀粉酶水平明显降低（P <0.05-0.01）。根据这些观察结果，肉眼检查和显微镜检查均显示，在所有观察到的时间点上，与模型对照相比，经BMSCs移植或G-CSF注射预处理的小鼠中SAP的病理变化明显减弱。结论：同种异体和动员自体骨髓间充质干细胞都可以保护小鼠胰腺免受SAP损伤。

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  • 来源
    《World Journal of Gastroenterology》 |2003年第10期|p.2274-2277|共4页
  • 作者

    Hui-Fei Cui; Zeng-Liang Bai;

  • 作者单位

    Biotechnological Institute of Materia Medica, Pharmaceutical College of Shandong University, Jinan 250012, Shandong Province, China;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 消化系及腹部疾病;
  • 关键词

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