...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>World Journal of Gastroenterology >Expression properties of recombinant pEgr-P16 plasmid in esophageal squamous cell carcinoma induced by ionizing irradiation
【24h】

Expression properties of recombinant pEgr-P16 plasmid in esophageal squamous cell carcinoma induced by ionizing irradiation

机译：重组pEgr-P16质粒在电离辐射诱导的食管鳞癌中的表达特性

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

AIM: To construct the recombinant pEgr-P16 plasmid for the investigation of its expression properties in esophageal squamous cell carcinoma induced by ionizing irradiation and the feasibility of gene-radiotherapy for esophageal carcinoma. METHODS: The recombinant pEgr-P16 plasmid was constructed and transfected into EC9706 cells with lipofectamine. Western blot, quantitative RT-PCR and flow cytpmetry were performed to study the expression of pEgr-P16 in EC9706 cells and the biological characteristics of EC9706 cell line after transfection induced by ionizing irradiation. RESULTS: The eukaryotic expression vector pEgr-P16 was successfully constructed and transfected into EC9706 cells. The expression of P16 was significantly increased in the transfected cells after irradiation while the transfected cells were not induced by ionizing irradiation. The induction of apoptosis in transfection plus irradiation group was higher than that in plasmid alone or irradiation alone. CONCLUSION: The combination of pEgr-P16 and irradiation could significantly enhance the P16 expression property and markedly induce apoptosis in EC9706 cells. These results may lay an important experimental basis for gene radiotherapy for esophageal carcinoma.
机译：目的：构建重组pEgr-P16质粒，研究其在电离辐射诱导的食管鳞状细胞癌中的表达特性，以及基因放射治疗食管癌的可行性。方法：构建重组质粒pEgr-P16，并用lipofectamine转染EC9706细胞。采用Western blot，定量RT-PCR和流式细胞仪研究电离辐射转染后EC9706细胞中pEgr-P16的表达及EC9706细胞的生物学特性。结果：成功构建了真核表达载体pEgr-P16，并转染到EC9706细胞中。辐照后转染的细胞中P16的表达显着增加，而电离辐射未诱导转染的细胞。转染加照射组细胞凋亡的诱导率高于单独质粒或单独照射组。结论：pEgr-P16与放射线联合使用可显着增强EC9706细胞的P16表达，并明显诱导其凋亡。这些结果可能为食管癌的基因放射治疗奠定重要的实验基础。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号