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首页> 外文期刊>World Journal of Gastroenterology >Down-regulation of gut-enriched Krueppel-like factor expression in esophageal cancer
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Down-regulation of gut-enriched Krueppel-like factor expression in esophageal cancer

机译：食管癌中富含肠道的Krueppel样因子表达下调

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AIM: Esophageal carcinoma is one of the most common malignant tumors in China. But the molecular mechanisms of esophageal carcinoma remains unclear. Gut-enriched Krueppel-like factor (GKLF) is a newly identified transcription factor which is expressed abandantly in the epithelial cells of the gastrointestinal tract and deregulation of GKLF was linked to several types of cancer. It is of interest to study the expression and role of GKLF in esophageal carcinoma. METHODS: Semi-quantitative RT-PCR was used to compare GKLF expression in esophageal squamous cell carcinoma to normal mucosa of the same patients. The serum deprivation inducibility of GKLF was observed in an esophageal squamous cancer cell line by comparison to the primary culture of human fibroblast. The effect of antisense GKLF transfection on the proliferation and adhesion of esophageal squamous cancer cell line was also observed. RESULTS: The level of GKLF transcript is lower in esophageal squamous cell carcinoma compared to paired normal-appearing mucosa in 14 of 17 of the tumors analyzed. The serum deprivation inducibility of GKLF was greatly decreased in an esophageal squamous cancer cell line compared to the primary culture of human fibroblast. Decreased expression of GKLF in the esophageal cancer cell by antisense GKLF transfection increased its proliferation rate compared with that of vector transfected cell control (P<0.05). Transfection of antisense GKLF decreased its adhesion ability (P<0.05). CONCLUSION: The findings of this study demonstrate the down-regulation of GKLF in esophageal squamous cancer, and suggest that deregulation of GKLF may play a role in initiation and/or progression as well as the metastasis of esophageal squamous cancer.
机译：目的：食管癌是中国最常见的恶性肿瘤之一。但是，食管癌的分子机制尚不清楚。富含肠的Krueppel样因子（GKLF）是一种新近鉴定出的转录因子，在胃肠道上皮细胞中大量表达，GKLF失调与多种类型的癌症有关。研究GKLF在食管癌中的表达及其作用是有意义的。方法：采用半定量RT-PCR技术比较同一患者食管鳞状细胞癌和正常黏膜中GKLF的表达。与人成纤维细胞的原代培养相比，在食管鳞状细胞癌细胞系中观察到了GKLF的血清剥夺诱导性。还观察到反义GKLF转染对食管鳞状细胞癌细胞系增殖和粘附的影响。结果：在所分析的17例肿瘤中，有14例的食管鳞状细胞癌中GKLF转录水平低于成对的正常黏膜。与人成纤维细胞的原代培养相比，食管鳞状细胞癌细胞系中GKLF的血清剥夺诱导性大大降低。与载体转染的细胞对照相比，反义GKLF转染降低了GKLF在食管癌细胞中的表达，提高了其增殖率（P <0.05）。反义GKLF的转染降低了其粘附能力（P <0.05）。结论：这项研究的结果证明了食管鳞状细胞癌中GKLF的下调，并暗示GKLF的失调可能在食管鳞状细胞癌的发生和/或发展以及转移中起作用。

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