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首页> 外文期刊>World Journal of Gastroenterology >p73β inhibits transcriptional activities of enhancer I and X promoter in hepatitis B virus more efficiently than p73α
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p73β inhibits transcriptional activities of enhancer I and X promoter in hepatitis B virus more efficiently than p73α

机译：p73β比p73α更有效地抑制乙型肝炎病毒中增强子I和X启动子的转录活性

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摘要

AIM: p73, as a novel member of a family of p53-related transcription factors, shares redundant functions with p53, such as the abilities of inducing apoptosis and suppressing growth. It is well known that p53 can repress HBV expression and transcription efficiently. The aim of this paper is to investigate the transcriptional effect of p73α and p73β on hepatitis B virus (HBV) and to understand the correlation between HBV and p73. METHODS: To construct an x-gene inactivated HBV plasmid which was cotransfected with p73α or p73β expression vectors into HepG2 cells. After transiently transfecticn, HBV surface antigen (HBsAg) and HBV e antigen (HBeAg) were detected by ELISA. Viral transcripts synthesized by HBV were evaluated by Northern blotting analysis. The activities of HBV regulatory elements, including enhancer I/X promoter (ENI/Xp) and enhancer II/core promoter (ENII/Cp) were monitored by luciferase assays. RESULTS: Both p73α and p73β could repress HBsAg and HBeAg expression by downregulating the ENI/Xp and ENII/ Cp activities. But p73β exerted stronger inhibition on the activity of ENI/Xp than p73α, resulting in much lower level of viral transcripts and the antigens expression. CONCLUSION: p73β as a novel member of p53 family can efficiently inhibit HBV transcription mainly through downregulating the activities of the HBV ENI/Xp regulatory elements.
机译：目的：p73，作为p53相关转录因子家族的新成员，与p53具有冗余功能，例如诱导细胞凋亡和抑制生长的能力。众所周知，p53可以有效抑制HBV的表达和转录。本文旨在研究p73α和p73β在乙型肝炎病毒（HBV）中的转录作用，并了解HBV与p73之间的相关性。方法：构建x基因灭活的HBV质粒，与p73α或p73β表达载体共转染到HepG2细胞中。瞬时转染后，通过ELISA检测HBV表面抗原（HBsAg）和HBV e抗原（HBeAg）。通过Northern印迹分析评估由HBV合成的病毒转录物。通过荧光素酶测定法监测包括增强子I / X启动子（ENI / Xp）和增强子II /核心启动子（ENII / Cp）的HBV调节元件的活性。结果：p73α和p73β均可通过下调ENI / Xp和ENII / Cp活性来抑制HBsAg和HBeAg的表达。但是，p73β对ENI / Xp的抑制作用比p73α强，导致病毒转录本和抗原表达水平大大降低。结论：p73β是p53家族的一个新成员，主要通过下调HBV ENI / Xp调节元件的活性来有效抑制HBV转录。

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  • 来源
    《World Journal of Gastroenterology》 |2002年第6期|p.1094-1097|共4页
  • 作者

    Zhen-Hua Xu; Mu-Jun Zhao; Tsai-Ping Li;

  • 作者单位

    State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 消化系及腹部疾病;
  • 关键词

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