• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>World Journal of Gastroenterology >Roles of Fas signaling pathway in vitamin E succinate-induced apoptosis in human gastric cancer SGC-7901 cells
【24h】

Roles of Fas signaling pathway in vitamin E succinate-induced apoptosis in human gastric cancer SGC-7901 cells

机译：Fas信号通路在维生素E琥珀酸酯诱导人胃癌SGC-7901细胞凋亡中的作用

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

AIM: To investigate the roles of Fas signaling pathway in vitamin E succinate-induced apoptosis in human gastric cancer SGC-7901 cells. METHODS: Human gastric cancer SGC-7901 cells were treated with VES at 5,10, 20 mg · L~(-1), succinic acid and vitamin E as vehicle control and condition media only as untreated (UT) control. Apoptotic morphology was observed by DAPI staining. Western blot analysis was applied to measure the expression of Fas, FADD and caspase-8 proteins. After the cells were transiently transfected with Fas and FADD antisense oligonucleotides, respectively, caspase-8 activity was determined by flurometric method. RESULTS: The morphologically apoptotic changes were observed after VES treatment by DAPI staining. 23.7 % and 89.6 % apoptosis occurred after 24 h and 48 h of 20 mg · L~(-1) VES treatment, respectively. The protein levels of Fas, FADD and caspase-8 were evidently increased in a dose-dependent manner after 24 h of VES treatment. The blockage of Fas by transfection with Fas antisense oligonucleotides obviously inhibited the expression of FADD protein. After SGC-7901 cells were transfected with Fas and FADD antisense oligonucleotides, caspase-8 activity was obviously decreased (P<0.01), whereas Fas blocked more than FADD. CONCLUSION: VES-induced apoptosis in human gastric cancer SGC-7901 cells involves Fas signaling pathway including the interaction of Fas, FADD and caspase-8.
机译：目的：探讨Fas信号通路在维生素E琥珀酸酯诱导人胃癌SGC-7901细胞凋亡中的作用。方法：以5,10,20 mg·L〜（-1）的VES，琥珀酸和维生素E作为媒介物对照和条件培养基作为未经处理的（UT）对照处理人胃癌SGC-7901细胞。通过DAPI染色观察到凋亡形态。蛋白质印迹分析用于测量Fas，FADD和caspase-8蛋白的表达。分别用Fas和FADD反义寡核苷酸瞬时转染细胞后，通过荧光法测定caspase-8活性。结果：DAPI染色后，VES处理后可见形态学上的凋亡变化。 20 mg·L〜（-1）VES处理24 h和48 h后，分别发生了23.7％和89.6％的细胞凋亡。 VES处理24小时后，Fas，FADD和caspase-8的蛋白水平明显呈剂量依赖性增加。 Fas反义寡核苷酸转染对Fas的阻断明显抑制了FADD蛋白的表达。用Fas和FADD反义寡核苷酸转染SGC-7901细胞后，caspase-8活性明显降低（P <0.01），而Fas的阻断作用大于FADD。结论：VES诱导的人胃癌SGC-7901细胞凋亡涉及Fas信号通路，包括Fas，FADD和caspase-8的相互作用。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号