首页> 外文期刊>World Journal of Gastroenterology >Killing effect of TNF-related apoptosis inducing ligand regulated by tetracycline on gastric cancer cell line NCI-N87
【24h】

Killing effect of TNF-related apoptosis inducing ligand regulated by tetracycline on gastric cancer cell line NCI-N87

机译:四环素调节的TNF相关凋亡诱导配体对胃癌细胞NCI-N87的杀伤作用

获取原文
获取原文并翻译 | 示例
           

摘要

AIM To clone the cDNA fragment of human TRAIL (TNF- related apoptosis inducing ligand) into a tetracycline- regulated gene expression system, the RevTet-On system, transduce expression vectors into a gastric carcinoma cell line-NCI-N87 and examine the effects of controlled expression of TRAIL in vitro on the gastric carcinoma cells. METHODS The full-length cDNA of TRAIL was inserted Into a vector under the control of the tetracycline- Responsive element (TRE) to obtain the plasmid pRevTRE- TRAIL, which was transfected into a packaging cell line PT67.
机译:目的将人TRAIL(TNF相关的凋亡诱导配体)的cDNA片段克隆到四环素调节的基因表达系统RevTet-On系统中,将表达载体转导到胃癌细胞系NCI-N87中,并研究其作用。在胃癌细胞中体外控制TRAIL的表达。方法在四环素反应元件(TRE)的控制下,将TRAIL的全长cDNA插入载体,获得质粒pRevTRE-TRAIL,将其转染到包装细胞系PT67中。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号