Bile acid formation in primary human hepatocytes

Curt Einarsson; Ewa Ellis; Anna Abrahamsson

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Bile acid formation in primary human hepatocytes

机译：人原代肝细胞中的胆汁酸形成

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AIM To evaluate a culture system for bile acid formation in primary human hepatocytes in comparison with HepG2 cells. METHODS Hepatocytes were isolated from Normal human liver tissue and were cultured in Serum-free William's E medium. The medium was Collected and renewed every 24 h. Bile acids and Their precursors in media were finally7 analysed by Gas chromatography-mass spectrometry.

机译：目的评估与HepG2细胞相比在原代人肝细胞中形成胆汁酸的培养系统。方法从正常人肝组织中分离肝细胞，并在无血清的William E培养基中培养。每24小时收集一次培养基并进行更新。最后用气相色谱-质谱法分析了培养基中的胆汁酸及其前体。

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来源
《World Journal of Gastroenterology》 |2000年第4期|p.522-525|共4页
作者
Curt Einarsson; Ewa Ellis; Anna Abrahamsson;
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收录信息美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
原文格式 PDF
正文语种 eng
中图分类消化系及腹部疾病;
关键词
bile acid formation; cell culture; cholesterol metabolism;

机译：胆汁酸形成;细胞培养;胆固醇代谢;

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1. Potency of individual bile acids to regulate bile acid synthesis and transport genes in primary human hepatocyte cultures [J] . LiuJ., LuH., LuY.-F., Toxicological sciences: An official journal of the Society of Toxicology . 2014,第2期

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2. Ethanol stimulates bile acid formation in primary human hepatocytes [J] . Nilsson LM, Sjovall J, Strom S, Biochemical and Biophysical Research Communications . 2007,第4期

机译：乙醇刺激人原代肝细胞中胆汁酸的形成
3. Primary cultures of human hepatocytes but not HepG2 hepatoblastoma cells are suitable for the study of glycosidic conjugation of bile acids [J] . E. Ellis, E. Roeb, H. -U. Marschall Biochimica et Biophysica Acta. Molecular and cell biology of Lipids . 2001,第2a3期

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4. Activation of a G-protein coupled receptor by conjugated bile acids in primary hepatocytes and liver: effects on glucose metabolism [C] . P. B. HYLEMON, Y. FANG, E. STUDER, International Bile Acid Meeting . 2007

机译：用原发性肝细胞和肝脏的共轭胆汁酸激活G-蛋白偶联受体：对葡萄糖代谢的影响
5. Vitamin D receptor regulation of cholesterol 7alpha-hydroxylase gene transcription and bile acid synthesis in human hepatocytes. [D] . Han, Shuxin. 2009

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6. Potency of Individual Bile Acids to Regulate Bile Acid Synthesis and Transport Genes in Primary Human Hepatocyte Cultures [O] . Jie Liu, Hong Lu, Yuan-Fu Lu, -1

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7. Chenodeoxycholic Acid Modulates Bile Acid Synthesis Independent of Fibroblast Growth Factor 19 in Primary Human Hepatocytes [O] . Helene Johansson, Jonas Nørskov Søndergaard, Carl Jorns, 2021

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Bile acid formation in primary human hepatocytes

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