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首页> 外文期刊>Bulletin of the National Research Centre >In vitro and in vivo Evaluation of the Genotoxicity of the Herbicide Glyphosate in Mice
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In vitro and in vivo Evaluation of the Genotoxicity of the Herbicide Glyphosate in Mice

机译：草甘膦除草剂对小鼠遗传毒性的体外和体内评价

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摘要

Cultured mouse spleen cells were exposed to the concentrations 10~(-3)-10~(-7)M glyphosate/ml medium, the full culture period 24 h. The percentage of viable cells reached 85.20% in the cultures treated with 10~(-7)M glyphosate ml/medium compared with 90% in the non-treated cultures. The concentrations 10~(-7), 10~(-5) and 5 x 10~(-5) M glyphosate/ml medium induced chromosome aberrations (P < 0.01) and SCE's in cultured mouse spleen cells in a dose dependent manner. It was found that glyphosate is a weak inducer of SCE's in vitro in cultured spleen cells and in vivo in bone marrow cells of the mouse. i.p injection with a single dose of 50 mg glyphosate kg~(-1) b.wt had no effect on chromosome aberrations either in bone-marrow cells or in spermatocytes. Multiple i.p. injection for 5 days with 50 mg glyphosate kg~(-1) b.wt induced 8.60 ± 0.64 and 7.80 ± 0.66 (P < 0.01) chromosome aberrations in bone marrow and spermatocyte cells, compared with 4.0 ± 0.50 and 3.60 ± 0.83 in somatic and germ cells of non-treated mice respectively. In another experiment, oral multiple treatment for 14 and 21 days with the doses 50 and 100 mg glyphosate kg~(-1) b.wt induced a significant increase in the percentage of chromosome aberrations. It reached 12.0 ± 1.12 and 10.20 ± 0.70 (P < 0.01) in somatic and germ cells respectively after oral treatment by gavage for 21 days with 100 mg glyphosate kg~(-1) b.wt. The doses, 100 and 200 mg glyphosate kg-1 b.wt, induced a dose dependent and significant (P < 0.05 and 0.01) increase in the percentage of sperm shape abnormalities. The results indicate that the herbicide glyphosate, N-(phosphono methyl) glycine, is genotoxic in mice under the circumstances tested.
机译：将培养的小鼠脾细胞暴露于浓度为10〜（-3）-10〜（-7）M草甘膦/ ml的培养基中，整个培养期为24 h。用10％（-7）M草甘膦ml /培养基处理的培养物中存活细胞的百分比达到85.20％，而未处理的培养物中的存活细胞百分比达到90％。浓度为10〜（-7），10〜（-5）和5 x 10〜（-5）的草甘膦/ ml培养基可诱导小鼠脾细胞中的染色体畸变（P <0.01）和SCE's呈剂量依赖性。发现草甘膦在体外在培养的脾细胞中和在小鼠的骨髓细胞中是SCE的弱诱导物。腹膜内注射单剂量50 mg草甘膦kg〜（-1）b.wt对骨髓细胞或精母细胞的染色体畸变没有影响。多次i.p.用50 mg草甘膦kg〜（-1）b.wt注射5天可诱导骨髓和精母细胞的染色体畸变为8.60±0.64和7.80±0.66（P <0.01），而体细胞为4.0±0.50和3.60±0.83和未处理小鼠的生殖细胞。在另一个实验中，以50和100 mg草甘膦kg〜（-1）b.wt的剂量口服多次治疗14天和21天，可导致染色体畸变百分比显着增加。用100 mg草甘膦kg〜（-1）b.wt口服管饲21天后，其在体细胞和生殖细胞中分别达到12.0±1.12和10.20±0.70（P <0.01）。草甘膦的剂量100和200 mg kg-1 b.wt引起精子形状异常百分比的剂量依赖性和显着（P <0.05和0.01）增加。结果表明，除草剂草甘膦N-（膦酰基甲基）甘氨酸在所测试的环境下对小鼠具有遗传毒性。

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