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首页> 外文期刊>Bulletin de la Societe Zoologique de France >UTILISATION EXPERIMENTALE ET ENVIRONNEMENTALE DES METALLOTHIONEINES HEPATIQUES EN TANT QUE BIOMARQUEUR CHEZ LE GARDON (RUTILUS RUTILUS)
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UTILISATION EXPERIMENTALE ET ENVIRONNEMENTALE DES METALLOTHIONEINES HEPATIQUES EN TANT QUE BIOMARQUEUR CHEZ LE GARDON (RUTILUS RUTILUS)

机译：肝金属硫蛋白作为甘草中的生物标志物的实验和环境用途

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Metallothioneins (MTs) show important induction of their synthesis when animals are exposed to metals. Consequently these proteins were proposed as biomarkers of metallic contamination and numerous studies investigate the impact of metals on MTs level in aquatic organisms. However aquatic ecosystems are frequently contaminated by a mix of pollutants: pesticides, heavy metals... There is a lack for information about the effect of pesticide contamination on MTs. So the aim of this work was to investigate experimentally and in environmental conditions the influence of pesticides on the MTs hepatic level of roach. Roach were exposed in laboratory conditions to sublethal concentrations of copper (40 or 140 μg/l) or procymidone (230 or 440 μg/l). Liver of roach exposed to copper showed obvious MTs induction (50 fold in roach exposed 7 d to 140 μg/l). However when the toxic impact of copper became more important as reaveled by histopathological approach (PARIS-PALACIOS et al., 2000b), the level of MTs decreased as fish entered the exhausted phase of stress. Procymidone contamination of roach liver induce very high synthesis of MTs (15 fold in roach exposed 4 d to 440 μg/l). For the first time, it has been shown that a pesticide can produce important induction of MTs. Thus MTs can constituted a non-specific biomarker of contaminant effect but not only a biomarker of exposure to metals. In order to validate MT levels as biomarkers in field studies, roach were also placed in two basins (B1 and B2) that receive the water coming from a vineyard at Reuil-sur-Marne (Champagne, France) and in a basin (B3) of the same site which is a flush of the alluvial water-table. B1 and B2 are contaminated by a mix of pollutants, particularly copper, diu-ron and procymidone; B3 contained no detactable amounts of pesticides and so was use as a reference. Roach was caged in these basins during 7 or 14 d at two different periods in marsh or april 2000. The analyse of hepatic MTs of caged roach showed that the evolution of hepatic MT level was correlated to the global contamination levels of each basin. Moreover the fluctuations of procymidone and diuron concentrations seemed to have a greater influence on MT levels than changes of Cu concentrations. We can conclude that MT levels can became interesting unspecific biomarkers to evaluate water quality in natural aquatic ecosystems.
机译：当动物接触金属时，金属硫蛋白（MTs）显示出重要的合成诱导作用。因此，这些蛋白质被提议作为金属污染的生物标志物，许多研究调查了金属对水生生物中MT含量的影响。但是，水生生态系统经常受到多种污染物的污染：农药，重金属...缺少关于农药污染对MT的影响的信息。因此，这项工作的目的是通过实验和在环境条件下研究农药对MTs肝脏蟑螂水平的影响。蟑螂在实验室条件下暴露于亚致死浓度的铜（40或140μg/ l）或嘧啶酮（230或440μg/ l）。暴露于铜的蟑螂肝脏表现出明显的MTs诱导（暴露7天至140μg/ l的蟑螂的50倍）。然而，当铜的毒性影响变得更为重要时，如通过组织病理学方法所揭示的那样（PARIS-PALACIOS等，2000b），随着鱼类进入压力的耗尽阶段，MTs的水平降低了。蟑螂肝脏中的嘧啶酮污染诱导了很高的MT合成（在蟑螂暴露4 d至440μg/ l时，其合成率是15倍）。首次证明农药可以产生重要的MT诱导。因此，MT可以构成污染效应的非特异性生物标记，而不仅是暴露于金属的生物标记。为了在野外研究中验证MT水平作为生物标记物，还将蟑螂放在两个盆地（B1和B2）中，该盆地接收来自Reuil-sur-Marne（法国香槟）葡萄园的水和一个盆地（B3）。同一地点的冲积水位齐平B1和B2被混合污染物污染，尤其是铜，铁隆和嘧啶酮。 B3不含可检测量的农药，因此可作为参考。在沼泽或2000年4月的两个不同时期，蟑螂被关在这些盆地中的7或14天。对笼养蟑螂肝脏MT的分析表明，肝脏MT水平的变化与每个盆地的总体污染水平相关。此外，嘧啶酮和杜隆浓度的波动似乎对MT水平的影响大于Cu浓度的变化。我们可以得出结论，MT水平可以成为评估自然水生生态系统中水质的有趣的非特异性生物标记。

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