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首页> 外文期刊>Cell Biochemistry and Biophysics >Effect of Zymosan on Antioxidant and Immune Function of S180 Tumor-Bearing Mice
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Effect of Zymosan on Antioxidant and Immune Function of S180 Tumor-Bearing Mice

机译：酵母聚糖对S 180 荷瘤小鼠抗氧化和免疫功能的影响

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The objective is to study the effect of zymosan on antioxidant and immune function of S180 tumor-bearing mice. Seventy Kunming mice were randomly divided into seven groups: a normal control group (NC), a tumor control group (TC), three dose groups of zymosan (low, medium, high), a cyclophosphamide (Cy) group, and a combination of zymosan and Cy group. The S180 tumor-bearing mice model was established by the inoculation of cancer cell suspension subcutaneously in the mouse’s right anterior limb. At the 19th day, malondialdehyde (MDA), glutathione peroxidase (GSH-Px), and superoxide dismutase (SOD) activity in liver homogenate were analyzed. The reverse transcriptase-polymerase chain reaction was used to determine the mRNA expression levels of IL-2, TNF-α, and TGF-β1. The activity of GSH-Px and SOD in the liver increased with the dose of zymosan, whereas the activity of MDA significantly decreased in the higher-dose groups of zymosan, compared to the TC group (P < 0.01). In the zymosan groups, mRNA expression levels in tissues of S180 tumor-bearing mice were significantly higher for TNF-α and IL-2, but lower for TGFβ1 than in the Cy or TC group (P < 0.01). The high-dose of zymosan markedly showed a depressant effect on S180 tumor, enhanced by the action of Cy that increased mRNA expression levels of TNF-α and IL-2. The mechanism of zymosan on the inhibition of tumor growth may be due to its ability to enhance the antioxidant and immune function in a dose-dependent manner.
机译：目的研究酵母聚糖对S 180 荷瘤小鼠抗氧化和免疫功能的影响。将70只昆明小鼠随机分为7组：正常对照组（NC），肿瘤对照组（TC），酵母聚糖3个剂量组（低，中，高），环磷酰胺（Cy）组以及下列药物的组合酵母聚糖和Cy组。通过在小鼠右前肢皮下接种癌细胞悬液，建立了S 180 荷瘤小鼠模型。在第19天，分析了肝匀浆中的丙二醛（MDA），谷胱甘肽过氧化物酶（GSH-Px）和超氧化物歧化酶（SOD）活性。用逆转录聚合酶链反应测定IL-2，TNF-α和TGF-β 1 的mRNA表达水平。肝脏中的GSH-Px和SOD活性随酵母聚糖剂量的增加而增加，而高剂量的酵母聚糖组与TC组相比，MDA的活性显着降低（P <0.01）。在酵母聚糖组中，S 180 荷瘤小鼠组织中的mRNA表达水平与TNF-α和IL-2相比明显升高，而与TGFβ 1 相比则降低。 Cy或TC组（P <0.01）。高剂量的酵母聚糖对S 180 肿瘤具有明显的抑制作用，而Cy的作用增强了TNF-α和IL-2的mRNA表达水平。酵母聚糖抑制肿瘤生长的机制可能是由于其以剂量依赖的方式增强抗氧化剂和免疫功能的能力。

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