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Characterization of 5'- proximal sequence of mouse GABA transporter gene (GAT-1)

机译：小鼠GABA转运蛋白基因（GAT-1）5'-近端序列的表征

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摘要

The cDNA molecule encoding the mouse GABA transporter gene (GAT-1) was used as probe for selecting GAT-1 gene from mouse genomic library. A positive clone, harboring the whole open reading frame of the GAT-1 protein and designated as MGABAT-G, was fished out from the library, the 5' proximal region and intron 1 were sequenced and analysed, and low homology was found in the above region between GAT-1 genes from mouse and human except some short conserved sequences. The DNA-protein interactions betweenDNA fragments containing the conserved sequences in the 5' proximal region and nuclear proteins from different tissues of mouse were studied by means of gel-shift assay, and Southern-Western blot. The results indicate a possible positive-negative regulation mode controlling the expression of the mouse GAT-1 gene.
机译：编码小鼠GABA转运蛋白基因（GAT-1）的cDNA分子被用作从小鼠基因组文库中选择GAT-1基因的探针。从文库中筛选出一个带有GAT-1蛋白完整开放阅读框并命名为MGABAT-G的阳性克隆，对5'近端区域和内含子1进行了测序和分析，结果发现该序列的同源性较低。除了一些短的保守序列之外，来自小鼠和人类的GAT-1基因之间的区域的上述区域。通过凝胶位移分析和Southern-Western印迹研究了5'近端区域中含有保守序列的DNA片段与小鼠不同组织的核蛋白之间的DNA-蛋白质相互作用。结果表明控制小鼠GAT-1基因表达的可能的正负调控模式。

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