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首页> 外文期刊>Cell and Tissue Research >Proliferative response of the stem cell system during regeneration of the rostrum in Macrostomum lignano (Platyhelminthes)
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Proliferative response of the stem cell system during regeneration of the rostrum in Macrostomum lignano (Platyhelminthes)

机译：木质大孢子（Platyhelminthes）母细胞再生过程中干细胞系统的增殖反应

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Macrostomum lignano (Platyhelminthes) possesses pluripotent stem cells, also called neoblasts, which power its extraordinary regeneration capacity. We have examined the cellular dynamics of neoblasts during regeneration of the rostrum in M. lignano. First, using live squeeze observations, the growth curve of the rostrum was determined. Second, neoblasts were labelled with 5-bromo-2'-deoxyuridine (BrdU) and an anti-phospho-histone H3 mitosis marker (anti-phos-H3) to analyze their proliferative response to amputation. During the regeneration process, both S- and M-phase cells were present anterior to the eyes, a region that is devoid of proliferating cells during homeostasis. Furthermore, BrdU pulse experiments revealed a biphasic S-phase pattern, different from the pattern known to occur during regeneration of the tail plate in M. lignano. During a first systemic phase, S-phase numbers significantly increased, both in the region adjacent to the wound (the anterior segment) and the region far from the wound (the posterior segment). During the second, spatially restricted phase, S-phase numbers in the anterior segment rose to a peak at 3 to 5 days post-amputation (p-a), while in the posterior segment, S-phase activity approached control values again. A blastema, characterized as a build-up of S- and M-phase cells, was formed 1 day p-a.
机译：木巨人（Platyhelminthes）拥有多能干细胞，也称为新生细胞，为其强大的再生能力提供了动力。我们已经检查了木质素中木质部再生过程中成神经细胞的细胞动力学。首先，利用实时挤压观察，确定讲台的生长曲线。其次，用5-溴-2'-脱氧尿苷（BrdU）和抗磷酸组蛋白H3有丝分裂标记物（anti-phos-H3）标记成胚细胞，以分析其对截肢的增殖反应。在再生过程中，S期和M期细胞均出现在眼前，在稳态过程中该区域没有增殖细胞。此外，BrdU脉冲实验显示了双相S相模式，这与已知的木质尾巴尾板再生过程中发生的模式不同。在第一系统性阶段期间，在与伤口相邻的区域（前段）和远离伤口的区域（后段）中，S期数量均显着增加。在第二个受空间限制的阶段中，前段的S期数字在截肢后3至5天（p-a）达到峰值，而在后段，S期的活性再次达到控制值。 1天p-a形成了以S期和M期细胞聚集为特征的胚细胞。

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