...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Cell and Tissue Research >Polymorphism of somatolactin-producing cells in the goldfish pituitary: immunohistochemical investigation for somatolactin-α and -β
【24h】

Polymorphism of somatolactin-producing cells in the goldfish pituitary: immunohistochemical investigation for somatolactin-α and -β

机译：金鱼垂体中产生生长激素的细胞的多态性：生长抑素-α和-β的免疫组织化学研究

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Somatolactin (SL) is a pituitary hormone belonging to the growth hormone/prolactin family of adenohypophyseal hormones. In teleost fish, SL is encoded by one or two paralogous genes, namely SL-α and -β. Our previous studies have revealed that pituitary adenylate-cyclase-activating polypeptide stimulates SL release from cultured goldfish pituitary cells, whereas melanin-concentrating hormone suppresses this release. As in other fish, the goldfish possesses SL-α and -β. So far, however, no useful means of detecting the respective SLs immunologically in this species has been possible. In order to achieve this aim, we raised rabbit antisera against synthetic peptide fragments deduced from the goldfish SL-α and -β cDNA sequences. Using these antisera, we observed adenohypophyseal cells showing SL-α- and -β-like immunoreactivities in the goldfish pituitary, especially the pars intermedia (PI). Several cells in the PI showed the colocalization of SL-α- and -β-like immunoreactivities. Then, using single-cell polymerase chain reaction with laser microdissection, we examined SL-α and -β gene expression in adenohypophyseal cells showing SL-α- or -β-like immunoreactivity. Among cultured pituitary cells, we observed three types of cell: those that possess transcripts of SL-α, -β, or both. These results suggest a polymorphism of SL-producing cells in the goldfish pituitary.
机译：生长抑素（SL）是一种垂体激素，属于腺垂体激素的生长激素/催乳激素家族。在硬骨鱼中，SL由一个或两个旁系基因编码，即SL-α和-β。我们以前的研究表明，垂体腺苷酸环化酶激活多肽可刺激培养的金鱼垂体细胞释放SL，而黑色素浓缩激素可抑制这种释放。与其他鱼类一样，金鱼拥有SL-α和-β。但是，到目前为止，尚没有有用的方法通过免疫学方法检测该物种中的各个SL。为了达到这个目的，我们提出了针对从金鱼SL-α和-βcDNA序列推导的合成肽片段的兔抗血清。使用这些抗血清，我们观察到垂体腺垂体细胞在金鱼垂体中，特别是在中间pars（PI）中显示出SL-α-和-β样免疫反应性。 PI中的几个细胞显示出SL-α-和-β-样免疫反应性的共定位。然后，使用单细胞聚合酶链反应和激光显微切割技术，我们检查了SL-α和-β基因在腺垂体细胞中的表达，显示出SL-α-或-β-样免疫反应性。在培养的垂体细胞中，我们观察到三种类型的细胞：具有SL-α，-β或两者转录本的细胞。这些结果表明在金鱼垂体中产生SL的细胞多态性。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号