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首页> 外文期刊>Cell and Tissue Research >Restart of steroidogenesis in dogs during recrudescence of testicular function following downregulation with a GnRH-agonist implant
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Restart of steroidogenesis in dogs during recrudescence of testicular function following downregulation with a GnRH-agonist implant

机译：用GnRH激动剂植入剂下调后，睾丸功能恢复过程中狗的类固醇生成重新开始

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摘要

To date, no details are available concerning the restart of steroidogenesis following the downregulation of testicular endocrine and germinative function by gonadotrophin-releasing hormone (GnRH)-agonist implants. This restart was assessed by determining the expression of steroidogenic acute regulatory (StAR) protein, cytochrome P450 side-chain cleavage enzyme (P450scc) and cytochrome P450 17α-hydroxylase,17,20-lyase (P450c17). The re-establishment of steroidogenesis was initiated by the removal of the GnRH-agonist implant (18.5 mg azagly nafarelin, Gonazon) at 5 months after treatment. Testes were removed at 3-week intervals (weeks 0–24) and four groups were formed according to the stage of spermatogenesis as revealed by the most developed germ cells observed (developmental group [DG] spermatocytes to DG elongated spermatids). Five dogs served as untreated controls. Positive immunostaining for StAR, P450scc and P450c17 was restricted to Leydig cells. Western blot indicated the specifity of the respective antibodies with hints of a expression of canine-specific P450scc and P450c17 proteins. A significant effect of group was observed for a percentage of the immunopositive area (PIA) as an indicator of active Leydig cells for StAR (P0.05), P450scc (P0.001) and P450c17 (P0.001), with PIA being lowest for the DG spermatocytes. With regard to the strength of the immunopositive signal, a significant effect of group was found for P450scc (P0.01) and P450c17 (P0.05), with the lowest intensity being observed in DG spermatocytes. At the mRNA level, the upregulation from DG spermatocytes to DG round spermatids was clearly evident but was only significant for P450scc (P0.05). Thus, downregulation affects the whole cascade of steroidogenesis, whereas withdrawal of inhibition results in a rapid restart, in part indicating a rebound phenomenon.
机译：迄今为止，关于促性腺激素释放激素（GnRH）激动剂植入物睾丸内分泌和生殖功能的下调后，类固醇生成的重新开始尚无任何详细信息。通过确定类固醇生成的急性调节（StAR）蛋白，细胞色素P450侧链裂解酶（P450scc）和细胞色素P45017α-羟化酶，17,20-裂合酶（P450c17）的表达来评估这种重启。在治疗后5个月，通过移除GnRH激动剂植入物（18.5 mg azagly nafarelin，Gonazon）来重新开始类固醇生成。每隔3周（0-24周）取出一次睾丸，根据观察到的最发达的生殖细胞（发育组[DG]精细胞到DG细长的精细胞），根据精子发生的阶段分为四组。五只狗作为未治疗的对照。 StAR，P450scc和P450c17的阳性免疫染色仅限于Leydig细胞。 Western印迹法显示了各自抗体的特异性，并暗示了犬特异性P450scc和P450c17蛋白的表达。观察到一组显着效果的免疫阳性面积（PIA）百分比指示了StAR（P <0.05），P450scc（P <0.001）和P450c17（P <0.001）的活跃Leydig细胞活性，PIA最低用于DG精母细胞。关于免疫阳性信号的强度，发现P450scc（P <0.01）和P450c17（P <0.05）的作用显着，在DG精母细胞中观察到最低的强度。在mRNA水平上，从DG精母细胞到DG圆形精子的上调是明显的，但仅对P450scc有意义（P <0.05）。因此，下调影响类固醇生成的整个级联，而撤消抑制作用导致快速重启，部分表明反弹现象。

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  • 来源
    《Cell and Tissue Research》 |2012年第3期|p.513-523|共11页
  • 作者

    M. Gentil; B. Hoffmann; A. Spang; K. Failing; S. Goericke-Pesch;

  • 作者单位

    Clinic for Obstetrics, Gynecology and Andrology of Large and Small Animals, Justus-Liebig-University Giessen, Frankfurter Strasse 106, 35392, Giessen, Germany;

    Clinic for Obstetrics, Gynecology and Andrology of Large and Small Animals, Justus-Liebig-University Giessen, Frankfurter Strasse 106, 35392, Giessen, Germany;

    Clinic for Obstetrics, Gynecology and Andrology of Large and Small Animals, Justus-Liebig-University Giessen, Frankfurter Strasse 106, 35392, Giessen, Germany;

    Unit for Biomathematics and Data Processing, Justus-Liebig-University Giessen, Frankfurter Strasse 95, 35392, Giessen, Germany;

    Clinic for Obstetrics, Gynecology and Andrology of Large and Small Animals, Justus-Liebig-University Giessen, Frankfurter Strasse 106, 35392, Giessen, Germany;

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  • 正文语种 eng
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  • 关键词

    Spermatogenesis; Downregulation; Steroidogenesis; StAR; P450scc; P450c17; Dog;

    机译：精子生成;下调;类固醇生成;StAR;P450scc;P450c17;狗;

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