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首页> 外文期刊>Cell and Tissue Research >Immunolocalization of aquaporin-4 in the brain, kidney, skeletal muscle, and gastro-intestinal tract of chicken
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Immunolocalization of aquaporin-4 in the brain, kidney, skeletal muscle, and gastro-intestinal tract of chicken

机译：Aquaporin-4在鸡脑，肾，骨骼肌和胃肠道中的免疫定位

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Aquaporin-4 (AQP4), a member of the water channel family, has high water permeability and multi-functional potentiality. Although an avian AQP4 homolog has recently been identified, its overall localization is still largely unknown. This study demonstrates the presence of AQP4 in several organs of chicken by using a specific chicken AQP4 antibody. Western blot analysis has revealed two bands of chicken AQP4 (30 and 32 kDa) in the brain, proventriculus, pectoral muscle, kidney, and ureter. The brain is the primary expression site of AQP4 in chicken. Immunohistochemical analysis of the brain has shown the highest AQP4 immunoreactivity around the cerebral ventricles, blood vessels, and the Purkinje cells. In peripheral organs, AQP4-immunoreactive elements have been observed in the ureter, glandular cells of the proventriculus, sarcolemma of the pectoral muscle, and the epithelium of the ceca and the rectum. Moreover, a heavily stained network of AQP4-immunoreactive fibers has been detected within the enteric plexuses.
机译：Aquaporin-4（AQP4）是水通道家族的成员，具有高透水性和多功能潜力。尽管最近发现了禽AQP4同源物，但其总体定位仍然未知。这项研究通过使用特定的鸡AQP4抗体证明了AQP4在鸡的几个器官中的存在。 Western blot分析显示，大脑，前胃，胸肌，肾脏和输尿管中有两条鸡AQP4谱带（30和32 kDa）。大脑是鸡中AQP4的主要表达位点。大脑的免疫组织化学分析显示，脑室，血管和浦肯野细胞周围的AQP4免疫反应性最高。在外周器官中，已在输尿管，前列腺小腺的腺细胞，胸肌的肌膜以及盲肠和直肠的上皮中观察到AQP4免疫反应性元件。此外，已在肠丛中检测到AQP4免疫反应性纤维的严重染色网络。

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