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首页> 外文期刊>Cell and Tissue Research >Steady-state level of epidermal growth factor (EGF) mRNA and effect of EGF on in vitro culture of caprine preantral follicles
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Steady-state level of epidermal growth factor (EGF) mRNA and effect of EGF on in vitro culture of caprine preantral follicles

机译：表皮生长因子（EGF）mRNA的稳态水平及其对山羊窦前卵泡体外培养的影响

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Our aim was to verify the steady-state level of epidermal growth factor (EGF) mRNA in goat follicles at various developmental stages and to investigate the influence of EGF on the survival, antrum formation and growth of secondary follicles cultured for 6 days. Primordial, primary and secondary goat follicles and small and large antral follicles were obtained to quantify EGF mRNA by real-time reverse transcription with the polymerase chain reaction. The influence of EGF and the presence or absence of follicle-stimulating hormone (FSH) on the development of secondary follicles and on mRNA expression for EGF and FSH receptor (FSH-R) was determined after 6 days of culture. Survival, antrum formation and follicular diameter were evaluated every other day of culture. EGF mRNA levels in secondary follicles were significantly higher than those in primordial follicles, whereas in small and large antral follicles, EGF mRNA levels in cumulus–oocyte complexes (COCs) were significantly higher than in granulosa/theca cells. During culture, EGF in the presence or absence of FSH increased the follicular daily growth rate of secondary follicles when compared with that in enriched alpha minimal essential medium. FSH, EGF or both reduced EGF mRNA levels, whereas EGF reduced FSH-R mRNA levels after follicle culture for 6 days. Thus, EGF mRNA levels are higher in secondary follicles than in earlier stages, with both FSH and EGF promoting the growth of goat secondary follicles. EGF and/or FSH reduce EGF mRNA levels, whereas EGF decreases FSH-R mRNA levels, in cultured secondary follicles.
机译：我们的目的是验证山羊卵泡在不同发育阶段的表皮生长因子（EGF）mRNA的稳态水平，并研究EGF对培养6天的次级卵泡的存活，胃窦形成和生长的影响。通过与聚合酶链反应的实时逆转录，获得了原始的，初级和次级的山羊卵泡以及小而大的肛门卵泡，以定量EGF mRNA。培养6天后，测定EGF和卵泡刺激素（FSH）的存在与否对次级卵泡发育以及对EGF和FSH受体（FSH-R）的mRNA表达的影响。每隔一天培养一次评估存活率，胃窦形成和卵泡直径。次级卵泡中的EGF mRNA水平显着高于原始卵泡，而在大小型的肛门卵泡中，卵丘-卵母细胞复合体（COC）中的EGF mRNA水平显着高于颗粒/卵泡膜细胞。在培养过程中，与富集的α基本必需培养基相比，在有或没有FSH的情况下，EGF均可提高次要卵泡的卵泡日生长速率。 FSH，EGF或两者均降低EGF mRNA水平，而EGF降低卵泡培养6天后的FSH-R mRNA水平。因此，次级卵泡中的EGF mRNA水平高于早期阶段，而FSH和EGF均促进山羊次级卵泡的生长。在培养的次级卵泡中，EGF和/或FSH降低EGF mRNA水平，而EGF降低FSH-R mRNA水平。

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