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首页> 外文期刊>Cell and Tissue Research >Sequential morphological characteristics of murine fetal liver hematopoietic microenvironment in Swiss Webster mice
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Sequential morphological characteristics of murine fetal liver hematopoietic microenvironment in Swiss Webster mice

机译：瑞士韦伯斯特小鼠的小鼠胎肝造血微环境的顺序形态学特征

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摘要

Embryonic hematopoiesis occurs via dynamic development with cells migrating into various organs. Fetal liver is the main hematopoietic organ responsible for hematopoietic cell expansion during embryologic development. We describe the morphological sequential characteristics of murine fetal liver niches that favor the settlement and migration of hematopoietic cells from 12 days post-coitum (dpc) to 0 day post-partum. Liver sections were stained with hematoxylin and eosin, Lennert’s Giemsa, Sirius Red pH 10.2, Gomori’s Reticulin, and Periodic Acid Schiff/Alcian Blue pH 1.0 and pH 2.5 and were analyzed by bright-field microscopy. Indirect imunohistochemistry for fibronectin, matrix metalloproteinase-1 (MMP-1), and MMP-9 and histochemistry for naphthol AS-D chloroacetate esterase (NCAE) were analyzed by confocal microscopy. The results showed that fibronectin was related to the promotion of hepatocyte and trabecular differentiation; reticular fibers did not appear to participate in fetal hematopoiesis but contributed to the physical support of the liver after 18 dpc. During the immature phase, hepatocytes acted as the fundamental stroma for the erythroid lineage. The appearance of myeloid cells in the liver was related to perivascular and subcapsular collagen, and NCAE preceded MMP-1 expression in neutrophils, an occurrence that appeared to contribute to their liver evasion. Thus, the murine fetal liver during ontogenesis shows two different phases: one immature and mainly endodermic (15 dpc) with the maturation of hepatocytes, a better definition of trabecular pattern, and an increase in the connective tissue in the capsule, portal spaces, and liver parenchyma. The decrease of hepatic hematopoiesis (migration) coincides with hepatic maturation.
机译：胚胎的造血作用是随着细胞迁移到各种器官中而动态发展而发生的。胎儿肝脏是负责胚胎发育过程中造血细胞扩增的主要造血器官。我们描述了从胎后12天（dpc）到产后0天有利于造血细胞沉降和迁移的鼠胎肝生态位的形态学序列特征。用苏木精和曙红，Lennert's Giemsa，Sirius Red pH 10.2，Gomori's网状蛋白和高碘酸席夫/阿辛蓝pH 1.0和pH 2.5对肝脏切片进行染色，并通过明场显微镜进行分析。共聚焦显微镜分析了纤连蛋白，基质金属蛋白酶-1（MMP-1）和MMP-9的间接免疫组织化学以及萘酚AS-D氯乙酸酯酶（NCAE）的组织化学。结果表明纤连蛋白与肝细胞的促进和小梁的分化有关。网状纤维似乎没有参与胎儿的造血作用，但在18 dpc后有助于肝脏的物理支持。在未成熟阶段，肝细胞充当红系谱系的基本基质。肝脏中髓样细胞的出现与血管周围和囊下胶原有关，NCAE在嗜中性粒细胞中先于MMP-1表达，这种现象似乎有助于其肝脏逃逸。因此，鼠胎儿肝脏在本体形成过程中显示出两个不同的阶段：一个是未成熟的且主要是内胚层（15 dpc），伴随着肝细胞的成熟，更好的小梁模式定义以及胶囊，门脉间隙和结缔组织中的结缔组织增加肝实质。肝造血功能减少（迁移）与肝成熟相吻合。

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