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首页> 外文期刊>Cell and Tissue Research >Proliferating cell nuclear antigen in gonad and associated storage tissue of the Pacific oyster Crassostrea gigas: seasonal immunodetection and expression in laser microdissected tissues
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Proliferating cell nuclear antigen in gonad and associated storage tissue of the Pacific oyster Crassostrea gigas: seasonal immunodetection and expression in laser microdissected tissues

机译：太平洋牡蛎Crassostrea gigas性腺和相关存储组织中增殖细胞核抗原：季节性免疫检测和激光显微切割组织中的表达。

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摘要

To understand the processes involved in tissue remodeling associated with the seasonal reproductive cycle of the oyster Crassostrea gigas, we used immunodetection and expression measurements of proliferating cell nuclear antigen (PCNA). The expression of the PCNA gene was measured by real-time polymerase chain reaction in the whole gonadal area compared with laser microdissected gonad and storage tissue. Results underlined the advantage of the laser microdissection approach to detect expression, mainly for early stages of spermatogenesis. In the storage tissue, PCNA expression was reduced in the gonadal tubules, but immunolabeled hemocytes and vesicular cells were detected when the storage tissue was being restored. In the gonadal tubules, the PCNA gene was more highly expressed in males than in females. As soon as spermatogenesis was initiated, PCNA expression showed a high and constant level. In females, the expression level increased gradually until the ripe stage. The immunological approach established the involvement of peritubular cells in gonadal tubule expansion during early gametogenesis. In both sexes, gonial mitosis was immunodetected throughout the reproductive cycle. In males, the occurrence of two types of spermatogonia was ascertained by differential immunolabeling, and intragonadal somatic cell proliferation was noted. As expected, immunolabeling was never observed from stage II spermatocytes to spermatozoa. In females, positively stained cells were detected from oogonia to growing oocytes with various labeled intracellular locations.
机译：为了了解与牡蛎Crassostrea gigas的季节性繁殖周期相关的组织重塑过程，我们使用了增殖细胞核抗原（PCNA）的免疫检测和表达测量。 PCNA基因的表达是通过实时聚合酶链反应在整个性腺区域与激光显微切割的性腺和储存组织进行比较而测得的。结果强调了激光显微解剖方法检测表达的优势，主要用于精子发生的早期阶段。在存储组织中，性腺小管中PCNA的表达降低，但是在恢复存储组织时检测到了免疫标记的血细胞和囊泡细胞。在性腺小管中，PCNA基因在男性中的表达高于女性。一旦开始生精，PCNA表达就显示出高水平和恒定水平。在雌性中，表达水平逐渐增加直至成熟阶段。免疫学方法建立了早期配子发生过程中肾小管周细胞参与性腺小管扩张的过程。男女均在整个生殖周期中检测到了有角的有丝分裂。在男性中，通过差异免疫标记确定了两种类型的精原细胞的发生，并且注意到了性腺内体细胞增殖。如所期望的，从未观察到从II期精细胞到精子的免疫标记。在雌性中，从卵小卵泡到生长中的带有不同标记细胞内位置的卵母细胞检测到阳性染色的细胞。

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