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首页> 外文期刊>Cell and Tissue Research >Expression of neuropeptide Y and agouti-related peptide in the hypothalamic arcuate nucleus of newborn neurogenin3 null mutant mice
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Expression of neuropeptide Y and agouti-related peptide in the hypothalamic arcuate nucleus of newborn neurogenin3 null mutant mice

机译：新生神经元3无效突变小鼠下丘脑弓状核中神经肽Y和刺骨相关肽的表达

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摘要

Mice deficient in neurogenin 3 (Ngn3) fail to generate pancreatic endocrine cells and intestinal endocrine cells. Hypothalamic neuropeptides implicated in the control of energy homeostasis might also be affected in Ngn3 homozygous null mutant mice. We investigated the expression of two prominent orexigenic neuropeptides, neuropeptide Y (NPY) and agouti-related protein (AgRP), in the hypothalamic arcuate nucleus of newborn wild-type and Ngn3 null mutant mice. Immunohistochemical analysis demonstrated that, in Ngn3 null mutants, the number of NPY-immunoreactive neurons and nerve fibers was markedly increased in the arcuate nucleus, and the nerve fibers were widely distributed in the hypothalamic area, including the paraventricular and dorsomedial nuclei. Little increase of AgRP immunoreactivity was detected in the arcuate nucleus of mutant mice. In situ hybridization analysis confirmed the increased population of the NPY neurons in the arcuate nucleus of the mutants. The NPY mRNA level, as estimated by laser capture microdissection and real-time quantitative polymerase chain reaction, was 371% higher in Ngn3 null mutants than in wild-type mice. AgRP mRNA levels did not differ significantly between the null mutants and wild-type mice. Thus, up-regulation of the hypothalamic NPY system is probably a feature characteristic of Ngn3 null mice.
机译：缺乏神经生成素3（Ngn3）的小鼠无法产生胰腺内分泌细胞和肠内分泌细胞。下丘脑神经肽参与能量稳态控制也可能在Ngn3纯合无效突变小鼠中受到影响。我们调查了两个野生动物和Ngn3 null突变小鼠的下丘脑弓形核中两个突出的致病性神经肽，神经肽Y（NPY）和刺骨相关蛋白（AgRP）的表达。免疫组织化学分析表明，在Ngn3无效突变体中，弓形核中NPY免疫反应性神经元和神经纤维的数量显着增加，并且神经纤维广泛分布在下丘脑区域，包括室旁和背侧核。在突变小鼠的弓形细胞核中，检测到的AgRP免疫反应性几乎没有增加。原位杂交分析证实了突变体弓形核中NPY神经元的数量增加。通过激光捕获显微切割和实时定量聚合酶链反应估计，NPY mRNA水平在Ngn3 null突变体中比野生型小鼠高371％。在无效突变体和野生型小鼠之间，AgRP mRNA水平没有显着差异。因此，下丘脑NPY系统的上调可能是Ngn3无效小鼠的特征。

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