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首页> 外文期刊>Cell and Tissue Research >Immunohistochemical characteristics of submucosal Dogiel type II neurons in rat colon
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Immunohistochemical characteristics of submucosal Dogiel type II neurons in rat colon

机译：大鼠结肠粘膜下Ⅱ级神经元神经元的免疫组织化学特征

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摘要

Secretory and motility reflexes are evoked by physiological stimuli in the isolated rat distal colon, which is therefore expected to contain intrinsic primary afferent (sensory) neurons. Dogiel type II neurons (putative intrinsic primary afferent neurons) exhibit several long processes emerging from large oval or round cell bodies. This study has examined the immunohistochemical characteristics of type II neurons in the submucosal plexus of rat distal colons by using whole-mount preparations. Neuronal cell bodies positive for both substance P (SP) and calretinin have been observed in colchicine-treated rats. Neurofilament 200 immunostaining has confirmed the type II morphology of SP-positive neurons. Moreover, all submucosal type II neurons identified by neurofilament 200 immunoreactivity are positive for calretinin. Calcitonin gene-related peptide (CGRP)-positive neurons in the submucosal plexus are distinct from type II neurons because they are negative for calretinin and have smaller cell bodies than the SP-positive submucosal type II neurons. Most (73%) of the submucosal neurons including type II neurons exhibit immunoreactivity for the neurokinin-1 receptor (NK1R), a receptor for SP, on the surface of cell bodies. Immunoreactivity for the EP3 receptor (EP3R), a receptor for prostaglandin E2, has been detected in 51% of submucosal neurons including type II neurons. Thus, submucosal type II neurons in the rat distal colon are immunopositive for SP/calretinin but immunonegative for CGRP. SP released from submucosal type II neurons probably acts via NK1Rs on type II and non-type II submucosal neurons to mediate intrinsic reflexes. EP3R-positive submucosal type II neurons may be potential targets of prostaglandin E2.
机译：分泌和运动反射是由离体大鼠远端结肠中的生理刺激引起的，因此预期其包含内在的初级传入（感觉）神经元。 Dogiel II型神经元（推定的内在原代传入神经元）表现出从大的卵形或圆形细胞体中出现的几个长过程。这项研究通过使用整装制剂检查了大鼠远端结肠粘膜下丛中II型神经元的免疫组织化学特征。在秋水仙碱治疗的大鼠中已观察到对物质P（SP）和钙网蛋白都呈阳性的神经元细胞体。 Neurofilament 200免疫染色已证实SP阳性神经元的II型形态。此外，通过神经丝200免疫反应鉴定出的所有粘膜下层II型神经元的钙网蛋白均为阳性。粘膜下丛中的降钙素基因相关肽（CGRP）阳性神经元不同于II型神经元，因为它们对Calretinin呈阴性，并且比SP阳性II型粘膜下神经元的细胞体要小。包括II型神经元在内的大多数（73％）粘膜下神经元在细胞体表面上对神经激肽-1受体（NK1R）（SP的一种受体）表现出免疫反应性。在包括II型神经元在内的51％粘膜下神经元中检测到了针对前列腺素E2的受体EP3受体（EP3R）的免疫反应性。因此，大鼠远端结肠中的粘膜下II型神经元对SP /钙黄蛋白免疫阳性，但对CGRP免疫阴性。从II型粘膜下神经元释放的SP可能通过NK1R作用于II型和非II型粘膜下神经元，以介导内在反射。 EP3R阳性II型粘膜下神经元可能是前列腺素E2的潜在靶标。

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