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首页> 外文期刊>Cell and Tissue Research >Immunoreactivity for high-affinity choline transporter colocalises with VAChT in human enteric nervous system
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Immunoreactivity for high-affinity choline transporter colocalises with VAChT in human enteric nervous system

机译：高亲和力胆碱转运蛋白与VAChT在人肠神经系统中共定位的免疫反应性

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摘要

Cholinergic nerves are identified by labelling molecules in the ACh synthesis, release and destruction pathway. Recently, antibodies against another molecule in this pathway have been developed. Choline reuptake at the synapse occurs via the high-affinity choline transporter (CHT1). CHT1 immunoreactivity is present in cholinergic nerve fibres containing vesicular acetylcholine transporter (VAChT) in the human and rat central nervous system and rat enteric nervous system. We have examined whether CHT1 immunoreactivity is present in nerve fibres in human intestine and whether it is colocalised with markers of cholinergic, tachykinergic or nitrergic circuitry. Human ileum and colon were fixed, sectioned and processed for fluorescence immunohistochemistry with antibodies against CHT1, class III beta-tubulin (TUJ1), synaptophysin, common choline acetyl-transferase (cChAT), VAChT, nitric oxide synthase (NOS), substance P (SP) and vasoactive intestinal peptide (VIP). CHT1 immunoreactivity was present in many nerve fibres in the circular and longitudinal muscle, myenteric and submucosal ganglia, submucosa and mucosa in human colon and ileum and colocalised with immunoreactivity for TUJ1 and synaptophysin confirming its presence in nerve fibres. In nerve fibres in myenteric ganglia and muscle, CHT1 immunoreactivity colocalised with immunoreactivity for VAChT and cChAT. Some colocalisation occurred with SP immunoreactivity, but little with immunoreactivity for VIP or NOS. In the mucosa, CHT1 immunoreactivity colocalised with that for VIP and SP in nerve fibres and was also present in vascular nerve fibres in the submucosa and on epithelial cells on the luminal border of crypts. The colocalisation of CHT1 immunoreactivity with VAChT immunoreactivity in cholinergic enteric nerves in the human bowel thus suggests that CHT1 represents another marker of cholinergic nerves.
机译：胆碱能神经通过标记ACh合成，释放和破坏途径中的分子来鉴定。最近，已经开发了针对该途径中另一种分子的抗体。突触中的胆碱重新摄取通过高亲和力胆碱转运蛋白（CHT1）发生。 CHT1免疫反应性存在于人与大鼠中枢神经系统和大鼠肠神经系统中的含有水泡乙酰胆碱转运蛋白（VAChT）的胆碱能神经纤维中。我们已经检查了人肠神经纤维中是否存在CHT1免疫反应性，以及它是否与胆碱能，速激肽或硝化回路的标志物共定位。使用针对CHT1，III类β-微管蛋白（TUJ1），突触素，普通胆碱乙酰基转移酶（cChAT），VAChT，一氧化氮合酶（NOS），P物质的抗体将人回肠和结肠固定，切片并进行荧光免疫组化处理SP）和血管活性肠肽（VIP）。 CHT1免疫反应性存在于人结肠和回肠的环状和纵向肌肉，肠系膜和粘膜下神经节，粘膜下层和粘膜的许多神经纤维中，并与TUJ1和突触素的免疫反应性共定位，从而确认其在神经纤维中的存在。在肌层神经节和肌肉的神经纤维中，CHT1免疫反应性与VAChT和cChAT的免疫反应性共定位。 SP免疫反应发生一些共定位，而VIP或NOS的免疫反应发生很少。在粘膜中，CHT1免疫反应性与VIP和SP在神经纤维中共定位，并且也存在于粘膜下层和隐窝腔边界上皮细胞的血管神经纤维中。因此，CHT1免疫反应性与VAChT免疫反应性在人肠的胆碱能肠神经中共定位，表明CHT1代表胆碱能神经的另一个标记。

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