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首页> 外文期刊>Cell and Tissue Research >Inflamed adult pharynx tissues and swimming larva of Ciona intestinalis share CiTNFα-producing cells
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Inflamed adult pharynx tissues and swimming larva of Ciona intestinalis share CiTNFα-producing cells

机译：成年的咽部发炎组织和Ciona intestinalis的游泳幼虫共享产生CiTNFα的细胞

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In situ hybridisation and immunohistochemistry analyses have shown that the Ciona intestinalis tumour necrosis factor alpha gene (CiTNFα), which has been previously cloned and sequenced, is expressed either during the inflammatory pharynx response to lipopolysaccharide (LPS) or during the swimming larval phase of development. Granulocytes with large granules and compartment/morula cells are CiTNFα-producing cells in both inflamed pharynx and larvae. Pharynx vessel endothelium also takes part in the inflammatory response. Haemocyte nodules in the vessel lumen or associated with the endothelium suggest the involvement of CiTNFα in recruiting lymphocyte-like cells and promoting the differentiation of inflammatory haemocytes. Specific antibodies against a CiTNFα peptide have identified a 43-kDa cell-bound form of the protein. Observations of pharynx histological sections (at 4 and 8 h post-LPS inoculation) from naive and medium-inoculated ascidians have confirmed the CiTNFα-positive tissue response. Larval histological sections and whole-mount preparations have revealed that CiTNFα is expressed by trunk mesenchyme, preoral lobe and tunic cells, indicating CiTNFα-expressing cell immigration events and an ontogenetic role.
机译：原位杂交和免疫组化分析表明，先前克隆和测序的Ciona肠肿瘤坏死因子α基因（CiTNFα）在对脂多糖的炎性咽部反应（LPS）或发育中的游泳幼虫期表达。在发炎的咽和幼虫中，具有大颗粒和室/桑mor细胞的粒细胞都是产生CiTNFα的细胞。咽部血管内皮也参与炎症反应。血管腔内或与内皮相关的血细胞结节提示CiTNFα参与募集淋巴细胞样细胞并促进炎症性血细胞分化。针对CiTNFα肽的特异性抗体已鉴定出该蛋白的43 kDa细胞结合形式。从幼稚和中度接种的海鞘观察咽组织学切片（LPS接种后4和8小时）已证实CiTNFα阳性组织反应。幼虫的组织学切片和整装制剂显示，CiTNFα在躯干间充质，口腔前叶和束缚细胞中表达，表明表达CiTNFα的细胞迁移事件和个体发育作用。

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