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首页> 外文期刊>Cell and Tissue Research >Anatomical and histological profiling of orphan G-protein-coupled receptor expression in gastrointestinal tract of C57BL/6J mice
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Anatomical and histological profiling of orphan G-protein-coupled receptor expression in gastrointestinal tract of C57BL/6J mice

机译：C57BL / 6J小鼠胃肠道中孤儿G蛋白偶联受体表达的解剖学和组织学分析

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摘要

G-protein-coupled receptors (GPCRs) constitute the largest family of transmembrane receptors and regulate a variety of physiological and disease processes. Although the roles of many non-odorant GPCRs have been identified in vivo, several GPCRs remain orphans (oGPCRs). The gastrointestinal (GI) tract is the largest endocrine organ and is a promising target for drug discovery. Given their close link to physiological function, the anatomical and histological expression profiles of benchmark GI-related GPCRs, such as the cholecystokinin-1 receptor and GPR120, and 106 oGPCRs were investigated in the mucosal and muscle-myenteric nerve layers in the GI tract of C57BL/6J mice by quantitative real-time polymerase chain reaction. The mRNA expression patterns of these benchmark molecules were consistent with previous in situ hybridization and immunohistochemical studies, validating the experimental protocols in this study. Of 96 oGPCRs with significant mRNA expression in the GI tract, several oGPCRs showed unique expression patterns. GPR85, GPR37, GPR37L1, brain-specific angiogenesis inhibitor (BAI) 1, BAI2, BAI3, and GPRC5B mRNAs were preferentially expressed in the muscle-myenteric nerve layer, similar to GPCRs that are expressed in both the central and enteric nerve systems and that play multiple regulatory roles throughout the gut-brain axis. In contrast, GPR112, trace amine-associated receptor (TAAR) 1, TAAR2, and GPRC5A mRNAs were preferentially expressed in the mucosal layer, suggesting their potential roles in the regulation of secretion, immunity, and epithelial homeostasis. These anatomical and histological mRNA expression profiles of oGPCRs provide useful clues about the physiological roles of oGPCRs in the GI tract.
机译：G蛋白偶联受体（GPCR）构成了最大的跨膜受体家族，并调节多种生理和疾病过程。尽管已在体内鉴定了许多无味GPCR的作用，但仍有数个GPCR仍然是孤儿（oGPCR）。胃肠道（GI）是最大的内分泌器官，并且是药物发现的有希望的目标。考虑到它们与生理功能的紧密联系，在胃肠道的粘膜和肌肉-肌层神经层中研究了基准胃肠道相关GPCR（例如胆囊收缩素-1受体和GPR120）和106种oGPCR的解剖学和组织学表达谱。 C57BL / 6J小鼠通过定量实时聚合酶链反应。这些基准分子的mRNA表达模式与以前的原位杂交和免疫组织化学研究一致，验证了本研究中的实验方案。在胃肠道中具有显着mRNA表达的96个oGPCR中，数个oGPCR显示出独特的表达模式。 GPR85，GPR37，GPR37L1，脑特异性血管生成抑制剂（BAI）1，BAI2，BAI3和GPRC5B mRNA在肌肉-肠系膜神经层中优先表达，类似于在中枢和肠神经系统中表达的GPCR相似，在整个肠脑轴中起着多种调节作用。相反，GPR112，微量胺相关受体（TAAR）1，TAAR2和GPRC5A mRNA在黏膜层中优先表达，表明它们在调节分泌，免疫和上皮稳态方面的潜在作用。 oGPCR的这些解剖和组织学mRNA表达谱提供了有关oGPCR在胃肠道中的生理作用的有用线索。

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