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首页> 外文期刊>Cell and Tissue Research >Immunofluorescence analysis of the internal brain anatomy of Nereis diversicolor (Polychaeta, Annelida)
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Immunofluorescence analysis of the internal brain anatomy of Nereis diversicolor (Polychaeta, Annelida)

机译:杂色Nereis(Polychaeta,Annelida)的大脑内部解剖结构的免疫荧光分析

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摘要

Comparative analyses of neuroanatomical characters can make valuable contributions to the inference of phylogenetic relationships. Whereas investigations in this field are numerous for arthropods, in-depth studies on other protostomes are sparse. Here, we provide a survey of the internal neuroarchitecture of the brain of the aciculate ragworm Nereis diversicolor (Polychaeta, Annelida). Descriptions are based on confocal laser scanning microscope analyses of brain sections labeled with the nuclear marker DAPI and antibodies raised against FMRF-amide, serotonin, and histamine. Autofluorescence of the nervous tissue has been utilized to further elucidate the anatomical structures of the brain. The architecture of two major brain compartments, i.e., the paired mushroom bodies and the central optic neuropil, is described in detail. The findings are compared with existent literature on polychaete neuroanatomy and on arthropod neuroanatomy, and possible phylogenetic implications are outlined.
机译:神经解剖学特征的比较分析可以为系统发育关系的推断做出宝贵的贡献。尽管在该领域对节肢动物的研究很多,但对其他原虫的深入研究却很少。在这里,我们提供了一种对小口worm虫Nereis diversicolor(Polychaeta,Annelida)的大脑内部神经体系结构的调查。描述基于共聚焦激光扫描显微镜对标记有核标记物DAPI的脑部切片以及针对FMRF-酰胺,5-羟色胺和组胺的抗体的分析。神经组织的自发荧光已被用来进一步阐明大脑的解剖结构。详细描述了两个主要脑室的结构,即成对的蘑菇体和中央视神经枕。将该发现与有关多poly类神经解剖学和节肢动物神经解剖学的现有文献进行比较,并概述了可能的系统发育意义。

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